Adult BALB/c mice (18-22 weeks, female, Charles River) were passively immunized with APA by the intravenous administration of mouse anti-PEG IgG1 (Silver Lake Research, CH2076, lot K0868) 24 hours prior to the intravenous administration of PLD (3 mg/kg, Doxil®, Janssen Products, LP). The administration of 30 μg of APA resulted in a plasma concentration of about 7 μg/mL at the time of PLD injection. In test groups, free PEG was administered intravenously (2,200 mg/kg for PEG 10 kDa, 550 mg/kg for PEG 20 kDa and 40 kDa) to mice 0.5 hours prior to the administration of PLD (3 mg/kg). Doses of PEG were administered and analyzed in mg/kg instead of μmole/kg in order to normalize for the number of repeating ethylene oxide backbone units regardless of PEG chain length. When technically possible, free PEG and PLD were administered into contralateral tail veins. At time points of 0.083, 3, 6, 24, 48, and 96 hours following PLD administration, mice were anesthetized using ketamine (100 mg/kg, i.p.) and medetomidine hydrochloride (1 mg/kg, i.p.), and sacrificed via cardiac puncture (into sodium heparin vacutainers) and cervical dislocation. The blood, liver, spleen, and lungs were collected for doxorubicin quantification.
Adult balb c mice
Manufactured by Charles River Laboratories
Sourced in United States, Japan, China
Adult BALB/c mice are a common inbred mouse strain used in biomedical research. These mice are characterized by their white coat color and susceptibility to certain diseases and conditions. They are often used as models for immunology, infectious disease, and cancer research.
Automatically generated - may contain errors
7 protocols using adult balb c mice
1
Passive Immunization and PEG Modulation of PLD Pharmacokinetics
2
Balb/c Mouse Welfare Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult ♂ Balb/c mice (Charles River, Wilmington, MA, USA) weighing 20–30 g were used. They had ad libitum access to food and water. All procedures were performed in accordance with the Canadian Council on Animal Care standards and Animal Research: Reporting In Vivo Experiments guidelines and were approved by the Animal Ethics Committee of Université Laval.
3
Embryonic and Postnatal Development in BALB/c Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BALB/c embryos and postnatal mice were used in the study. The examined embryonic and postnatal stages were E10.5, E12, E13, E14, E15, E16, E17 and E18 after gestation and postnatal days 0 (PN0), PN1, PN2, PN3, PN4, PN5, PN10 and PN20. Adult BALB/c mice were obtained from Charles River Japan Inc. (Yokohama, Japan). All experimental procedures using mice were approved by the Animal Care and Use Review Committee, Kyushu University (Fukuoka, Japan) and performed according to the guidelines of the committee. After allowing 12 hr for the mating of 10–20 week-old-female and adult male BALB/c mice, the presence of successful insemination was determined based on the presence of a post-copulatory plug in the vagina, and this time point was defined as E0.5 [14] (link), [16] (link), [17] (link).
4
SLE Disease Progression in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MRL/MpJ-Faslpr/J (will be referred to as MRL/Lpr throughout the manuscript) and MRL/MpJ (will be referred to as MRL throughout the manuscript) mice, were purchased from The Jackson Laboratory (Bar Harbor, ME). Adult BALB/c mice were purchased from Charles River Laboratories (Wilmington, MA). Mouse experiments were approved by the US Food and Drug Administration/Center for Biologics Evaluation and Research Institutional Animal Care and Use Committee (permit number 2002–31). As approved in the protocol, mice showing advanced SLE disease symptoms such as severe skin lesions characterized by >5% of the skin surface, stress symptoms manifested with increased respiratory rate with an abdominal component in breathing, loss of response to external stimuli, and severe lymphadenopathy characterized by easily visible lymph nodes interfering with mobility were euthanized early as a humane endpoint.
5
Balb-c Mice Organ Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice imaging was approved by the Institutional Animal Care and Use Committee of University of Southern California, protocol no. 20847. Experimental research on vertebrates complied with institutional, national and international ethical guidelines. Animals were kept on a 12-h light–dark cycle. Animals were breathing double filtered air, temperature in the room was kept at 70–73 °F, with humidity at 50% and cage bedding was changed biweekly. All these factors contributed to minimize intra- and inter-experiment variability. Adult Balb-c mice (Charles River Laboratories) were killed via overdose of isoflurane followed by cervical dislocation. Organs were quickly collected from the mice, washed in phosphate-buffered saline (PBS) and cut longitudinally alongside the mid-section to expose the inner part of the organ. The two halves of the organ were arranged onto a microscope slide for imaging.
6
Mouse Model Husbandry and Handling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All procedures were approved by the Johns Hopkins University Animal Care and Use Committee. Female or male adult BALB/c mice were purchased from Charles River Laboratories. Uninfected mice used for PK studies were maintained in animal biosafety level 2 (ABSL-2) facilities, and infected mice used for PK/PD studies were maintained in ABSL-3 facilities. All mice were housed in individually ventilated cages (up to five mice per cage) with access to food and water ad libitum and with sterile shredded paper for bedding. Room temperature was maintained at 22 to 24°C, and a 12-h light/12-h dark cycle was used. All mice were killed by intentional isoflurane overdose by inhalation (drop method) followed by cervical dislocation.
7
Animal Husbandry and Experimental Procedures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult BALB/c mice (20 g) and adult Wistar rats (200 g) were obtained from the Charles River Laboratories (Beijing, China). All procedures were performed in accordance with the guiding principles in the Care and Use of Animals and approved by the Capital Medical University Committee on the Use of Animals in Research and Education. Animals were separately housed in plastic cages in a room maintained at 23.6°C and 35% humidity with 12-hour light/dark cycles (light on at 07:00 AM). Each animal was used only once and fed a standard chow diet with unrestricted water intake. Experiments were conducted in an ABSL-2 laboratory and at the end of the experiments, the animals were anesthetized using pentobarbital sodium and then euthanized.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!