Docetaxel was purchased from ScinoPharm Taiwan (Tainan, Taiwan). Soybean lecithin (S 100) was provided by Lipoid (Ludwigshafen, Germany). 1.2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) and 1.2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG5000) were purchased from NOF (Tokyo, Japan). Kolliphor® TPGS was obtained from BASF (Florham Park, NJ, USA). 3.30-Dioctadecyloxacarbocyanine perchlorate (DIO) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Tynen® (docetaxel), a generic product, was provided by TYY Pharmaceutical (Taipei, Taiwan). Fetal bovine serum, non-essential amino acids and sodium pyruvate were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Dulbecco’s Modified Eagle Medium (DMEM), Minimum Essential Medium (MEM) and penicillin/streptomycin/amphotericin B solution were purchased from Corning Incorporated (Corning, NY, USA). Other reagents and solvents were of analytical grade. WS1, MIA PaCa-2 and HT-29 were supplied by American Type Culture Collection (Manassas, VA, USA). WS1/FAP cells were human WS1 fibroblasts engineered to overexpress human FAP by lentiviral infection.
Penicillin streptomycin amphotericin b solution
Manufactured by Corning
Sourced in United States
Penicillin/streptomycin/amphotericin B solution is a sterile, liquid mixture of antibiotics and an antifungal agent commonly used in cell culture applications. The solution contains defined concentrations of penicillin, streptomycin, and amphotericin B to prevent bacterial and fungal contamination in cultured cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Soybean lecithin s100, by Lipoid (1 mentions)
Kolliphor tpgs, by BASF (1 mentions)
Lymphocyte separation medium, by Corning (1 mentions)
Cyclosporine a, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlas Biologicals (1 mentions)
L glutamine, by Corning (1 mentions)
2 protocols using penicillin streptomycin amphotericin b solution
1
Docetaxel Nanoparticle Formulation and Cell Lines
2
Lymphocyte Isolation and Immortalization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Blood obtained from patients DGDP289A, DGDP289B and other family members (Figure 1 A) was used to isolate lymphocytes by density gradient centrifugation. From patient’s whole blood samples, lymphocytes were isolated using density gradient centrifugation. Ten mL of the blood sample was layered on top of the 5 mL of Lymphocyte Separation Medium (Cellgro, Manassas, VA, USA) in 15 mL tubes, and the tubes were centrifuged at 400× g for 30 min. From the tubes, the lymphocyte layer in the middle was carefully removed and washed in PBS (Fisher, Pittsburgh, PA, USA) twice at 300× g for 10 min. Then, the lymphocytes were immortalized by adding cyclosporine A (Sigma, St. Louis, MO, USA) and Epstein–Barr virus (EBV) to the cell pellet, and maintained in RPMI (Hyclone, South Logan, Australia) containing 20% fetal bovine serum (Atlas Biologicals, Fort Collins, CO, USA), 1% l- glutamine (Corning, Corning, NY, USA), and 1% penicillin/streptomycin/amphotericin B solution (Corning, Corning, NY, USA) [26 (link)].
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