Log In Sign up
The largest database of trusted experimental protocols

Trypsin 0.25

Manufactured by Merck Group
Visit Supplier
Sourced in Spain, United States

Trypsin (0.25%) is a proteolytic enzyme commonly used in cell culture and molecular biology applications. It functions to dissociate adherent cells from a substrate or culture surface. Its core purpose is to facilitate the harvesting and passaging of cells.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Merck Group (2 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Atorvastatin, by Merck Group (1 mentions) Celltiter 96 aqueous non radioactive cell proliferation assay, by Promega (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Penicillin streptomycin solution, by Merck Group (1 mentions) D glucose, by AppliChem (1 mentions) L glutamine, by Merck Group (1 mentions) Dulbecco s modified eagle s medium, by Merck Group (1 mentions) Dulbecco s phosphate buffered saline, by Merck Group (1 mentions) Nih3t3, by American Type Culture Collection (1 mentions) βhb assay kit, by Merck Group (1 mentions)

Spelling variants of this product

Trypsin (3740 citations) Trypsin-EDTA 0.25% (32 citations) Trypsin-EDTA solution 0.25% (19 citations) Trypsin-ethylenediaminetetraacetic acid 0.25% (2 citations)

4 protocols using trypsin 0.25

1

Angiotensin II and Atorvastatin Effects on HUVECs

Check if the same lab product or an alternative is used in the 5 most similar protocols
HUVECs (obtained from the Cell Bank of the Chinese Academy of Sciences, Shanghai, China) were cultured in Dulbecco's modified Eagle's medium (DMEM; Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) containing 10% fetal bovine serum (FBS; Hyclone; GE Healthcare Life Sciences) and penicillin-streptomycin (100 U/ml-100 µg/ml; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), in humidified atmosphere with 5% CO2 at 37°C. Medium was replaced every 2 days or as necessary. For each experiment, the cells were treated with 1 µM Ang II alone or in combination with 10 µM atorvastatin (both from Sigma-Aldrich; Merck KGaA) for 24 h. Trypsin (0.25%) (Sigma-Aldrich; Merck KGaA) was used for digestion.
Chang Y., Li Y., Ye N., Guo X., Li Z., Sun G, & Sun Y. (2017). Atorvastatin protects the proliferative ability of human umbilical vein endothelial cells inhibited by angiotensin II by changing mitochondrial energy metabolism. International Journal of Molecular Medicine, 41(1), 33-42.
+ Open protocol
+ Expand
2

Cytotoxicity Assay Using Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture media (McCoy’s 5A and DMEM), FBS (fetal bovine serum), trypsin (0.25%), penicillin-streptomycin solution, and DMSO (dimethyl sulfoxide) were purchased from Sigma-Aldrich (Barcelona, Spain). D-glucose was acquired from AppliChem (Barcelona, Spain). CellTiter 96®AQueous Non-Radioactive Cell Proliferation Assay (used for the cellular viability assays) was purchased from Promega (Lisbon, Portugal).
Silva P.M., Pinheiro P.F., Camões S.P., Ribeiro A.P., Martins L.M., Miranda J.P, & Justino G.C. (2023). Exploring the Mechanisms behind the Anti-Tumoral Effects of Model C-Scorpionate Complexes. Molecules, 28(14), 5451.
+ Open protocol
+ Expand
3

Oregano Byproduct Reinforced Polymer Composites

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oregano byproduct was kindly
provided by Kütaş Tarım Ürünleri
Dış Tic. Ve San A.Ş., Turkey. Poly(propylene carbonate)
(PPC) was purchased from Empower Materials, U.S.A. Cellulose acetate
(CA), 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH•), Dulbecco’s modified Eagle’s medium (DMEM), Fetal
Bovine Serum (FBS), l-Glutamine (200 mM), Trypsin 0.25% (containing
1 mM EDTA), Water-Soluble Tetrazolium Salt (WST-1) assay and Dulbecco’s
Phosphate Buffered Saline (DPBS), acetone and ethanol were obtained
from Sigma-Aldrich, Italy. All chemicals were analytical grade and
used as received without any further purification. Deionized water
was obtained from Milli-Q Advantage A10 ultrapure water purification
system. NIH/3T3 (murine fibroblasts cell line) was received from American
Type Culture Collection (ATCC).
Tran T.N., Mai B.T., Setti C, & Athanassiou A. (2020). Transparent Bioplastic Derived from CO2-Based Polymer Functionalized with Oregano Waste Extract toward Active Food Packaging. ACS Applied Materials & Interfaces, 12(41), 46667-46677.
+ Open protocol
+ Expand
4

Quantifying Intracellular β-HB in SW 1353 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
SW 1353 cells were detached with trypsin 0.25 % (Sigma-Aldrich) and used to quantify intracellular β-HB production using the β-HB Assay Kit (MAK041 Sigma-Aldrich).
Vargas-López M., Quiroz-Vicente C.A., Pérez-Hernández N., Gómez-Chávez F., Bañuelos-Hernández A.E, & Pérez-Hernández E. (2024). The ketone body β-Hydroxybutyrate as a fuel source of chondrosarcoma cells. Heliyon, 10(9), e30212.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!