Digimizer image analysis software

Hip MRI was performed with a 3.0-T MR scanner (Magnetom Trio with TIM System;
Siemens Healthcare) and a dedicated flexible surface coil around the affected
hip joint, as described by Gao et al.^{7 (link)}
The preoperative alpha angle and lateral center-edge angle (LCEA) were
measured on the 45° Dunn view and supine anteroposterior hip radiographs,
respectively, as described in previous studies.^{1 (link),3 (link)}
The first author (G.G.), who had >5 years of experience in hip MRI and
arthroscopy, analyzed all images and was blinded to the clinical and operative
findings. Based on the LCEA measurement, the patients were divided into 3
groups: BDDH group (20°-25°), control group (25°-40°), and pincer group
(>40°) (Figure
1).
The cross-sectional areas of the IC and the RF were measured preoperatively and
postoperatively on a single MRI axial slice at the level of the femoral head
center as described by Haefeli et al^{10 (link)}
(Figure 2). The
measurements were performed by 2 evaluators (G.G. and Y.X.). The outlines of
both the IC and RF were determined manually, and the cross-sectional area of
each was calculated using Digimizer Image Analysis software (Version 4.5.2;
MedCalc Software Ltd). The IC-to-RF ratio was calculated by dividing the IC
cross-sectional area by the RF cross-sectional area. We randomly selected 30
scans to evaluate interrater reliability.

Thyroid follicle area measurements were performed on digital images of hematoxylin and eosin–stained thyroid tissue sections using the Digimizer Image Analysis software (MedCalc Software Ltd, Ostend, Belgium). A closed loop was drawn around individual thyroid follicles, and the area was calculated by the software using the length and width measurements (Fig. S5 (26 )). For each thyroid gland, measurements from 100 follicles were collected. Five animals were analyzed per group.