Xanthine oxidase fluorometric assay kit

Manufactured by Cayman Chemical

Sourced in United States

The Xanthine Oxidase Fluorometric Assay Kit is a tool used to quantify the activity of the enzyme xanthine oxidase. It utilizes a fluorometric detection method to measure the oxidation of a substrate, providing a sensitive and quantitative assessment of xanthine oxidase levels.

Automatically generated - may contain errors

Lab products found in correlation

Ua assay kit, by Cayman Chemical (1 mentions) Versamax, by Molecular Devices (1 mentions) Allopurinol, by Merck Group (1 mentions) Rabbit anti glut9 antibody, by Merck Group (1 mentions)

Close spelling variants of this product

Fluorometric assay kit

6 protocols using xanthine oxidase fluorometric assay kit

Hypoxia-Induced Oxidative Stress Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hypoxia induced oxidative stress was evaluated using enzymatic and non-enzymatic cellular anti-oxidants i.e. free radical generation by ROS estimation, GSH/GSSH, lipid peroxidation and xanthine oxidase assays at different time points of 1, 3, 6, 12 and 24 h of hypoxia by quantitative methods.
Free radical generation was assessed by the method described by Cathcart et. al. [48 (link)]. Reduced glutathione (GSH) and oxidised glutathione (GSSG) levels in cytosolic fractions were determined fluorimetrically by the method of Hissin and Hilf [49 (link)]. Lipid peroxidation was measured in cellular cytosolic fraction in terms of formation of malondialdehyde as described by Utley et. al. [50 (link)]. To evaluate generation of superoxide radicals under hypoxia, assessment of xanthine oxidase activity was done in cytosolic fractions of HVCM cells using Xanthine Oxidase Fluorometric assay kit (Cayman Chemical, 10010895) according to manufacturer’s instructions.

Nehra S., Bhardwaj V., Ganju L, & Saraswat D. (2015). Nanocurcumin Prevents Hypoxia Induced Stress in Primary Human Ventricular Cardiomyocytes by Maintaining Mitochondrial Homeostasis. PLoS ONE, 10(9), e0139121.

+ Open protocol

+ Expand

Uricase Inhibition Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The XOD inhibitor AP and uricase inhibitor potassium oxonate (PO; or oxonic acid potassium salt) were purchased from Sigma (St. Louis, MO, USA). The UA assay kit and Xanthine Oxidase Fluorometric Assay Kit was purchased from Cayman Chemical Company (Ann Arbor, MI, USA).

Chen R.J., Chen M.H., Chen Y.L., Hsiao C.M., Chen H.M., Chen S.J., Wu M.D., Yech Y.J., Yuan G.F, & Wang Y.J. (2016). Evaluating the urate-lowering effects of different microbial fermented extracts in hyperuricemic models accompanied with a safety study. Journal of Food and Drug Analysis, 25(3), 597-606.

+ Open protocol

+ Expand

Liver Xanthine Oxidase Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

After the livers were homogenized and centrifuged, the supernatant was collected. The xanthine oxidase activities were detected using a xanthine oxidase fluorometric assay kit (Cayman Chemical, Ann Arbor, MI, USA). The procedures were followed according to the manufacturer’s instructions.

Chau Y.T., Chen H.Y., Lin P.H, & Hsia S.M. (2019). Preventive Effects of Fucoidan and Fucoxanthin on Hyperuricemic Rats Induced by Potassium Oxonate. Marine Drugs, 17(6), 343.

+ Open protocol

+ Expand

Inflammatory Biomarkers Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

ELISA kits were used to measure the serum and kidney inflammatory factor index IL-1β (Biovision, K4796-100) and the Xanthine Oxidase Fluorometric Assay Kit (Cayman Chemical, Ann Arbor, MI, USA) in the liver. The manufacturer’s instruction was followed and a VERSA Max microplate reader was used for measurement (Molecular Devices, San Jose, CA, USA).

Chang Y.H., Chiang Y.F., Chen H.Y., Huang Y.J., Wang K.L., Hong Y.H., Ali M., Shieh T.M, & Hsia S.M. (2021). Anti-Inflammatory and Anti-Hyperuricemic Effects of Chrysin on a High Fructose Corn Syrup-Induced Hyperuricemia Rat Model via the Amelioration of Urate Transporters and Inhibition of NLRP3 Inflammasome Signaling Pathway. Antioxidants, 10(4), 564.

+ Open protocol

+ Expand

Measuring Adenosine Metabolism in BALF

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adenosine metabolism in BALF was assessed by measuring hypoxanthine generated after incubating 5 µL BALF supernatant with 200 µM adenosine in 50 µL Tris pH 7.5 at 37°C for one hour. Resulting hypoxanthine was assessed using a reaction mix containing Amplex red, horseradish peroxidase, and xanthine oxidase from the Xanthine Oxidase Fluorometric Assay Kit (Cayman Chemicals, Ann Arbor, MI). Signal was measured on a fluorometric plate reader with excitation 530 nM/absorbance 570 nM.

Esther CR J.r., Turkovic L., Rosenow T., Muhlebach M.S., Boucher R.C., Ranganathan S, & Stick S.M. (2016). Metabolomic biomarkers predictive of early structural lung disease in cystic fibrosis. The European respiratory journal, 48(6), 1612-1621.

+ Open protocol

+ Expand

Laminaria japonica-Derived Bioactives for Gout

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hi-Q oligo-fucoidans (Fc) and high-stability fucoxanthin (HS Fucoxanthin, HSFUCO) (Fx) were derived from Laminaria japonica and obtained from Hi-Q Marine Biotech International Ltd. (New Taipei City, Taiwan) [27 (link)]. Oxonic acid (potassium salt), hypoxanthine, and the xanthine oxidase fluorometric assay kit were purchased from Cayman Chemical (Ann Arbor, MI, USA). Allopurinol was purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-ABCG2 and anti-OAT1 antibodies were obtained from Abcam (Cambridge, MA, USA). Rabbit anti-GLUT9 antibody was obtained from Millipore (Billerica, MA, USA), and rabbit anti-URAT1 antibody was obtained from Proteintech (Rosemont, IL, USA).

Chau Y.T., Chen H.Y., Lin P.H, & Hsia S.M. (2019). Preventive Effects of Fucoidan and Fucoxanthin on Hyperuricemic Rats Induced by Potassium Oxonate. Marine Drugs, 17(6), 343.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!