Embryos and larvae were fixed in 4% PFA (pH 7.3) over night at 4 °C and stored in PBS containing 0.1% Tween 20 at 4 °C until use. WIF experiments were performed essentially as described33 (link) using the following antibodies: rabbit anti-T4 polyclonal antibody (1:2000; MP Biochemicals), chicken anti-GFP polyclonal antibody (1:1000; Abcam), rabbit anti-pax2a polyclonal antibody (1:250; GeneTex), cy3-conjugated donkey anti-rabbit IgG antibody (1:250; Jackson ImmunoResearch), and Alexa Fluor 488-conjugated goat anti-chicken IgG antibody (1:250; Invitrogen). Stained specimens were postfixed in 4% PFA, gradually transferred to 100% glycerol and phenotypically analysed using a M165 FC fluorescence stereomicroscope. Whole mount imaging of stained specimens was performed with a DMI600B epifluorescence microscope equipped with a DFC365FX camera.
Alexa fluor 488 conjugated goat anti chicken igg antibody
Manufactured by Thermo Fisher Scientific
The Alexa Fluor 488-conjugated goat anti-chicken IgG antibody is a fluorescently labeled secondary antibody. It is designed to detect and bind to chicken immunoglobulin G (IgG) antibodies in various immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Cy3 conjugated donkey anti rabbit igg antibody, by Jackson ImmunoResearch (1 mentions)
Chicken polyclonal anti gfp antibody, by Abcam (1 mentions)
Chicken polyclonal anti gfp, by Abcam (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Fluoromount g, by Southern Biotech (1 mentions)
Draq5, by Biostatus (1 mentions)
2 protocols using alexa fluor 488 conjugated goat anti chicken igg antibody
1
Whole-Mount Immunofluorescence Staining of Zebrafish Embryos
2
Immunohistochemistry for GFP and reporter proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissue was isolated, fixed, sectioned, and permeabilized as described above. Tissue was blocked in 10% normal goat serum in PBS for 1 h, followed by an overnight incubation at 4°C with chicken polyclonal anti-GFP (1:2500 dilution; catalog 13970; Abcam). Following washes, sections were incubated with Alexa Fluor-488-conjugated goat anti-chicken IgG antibody (1:2000 dilution; Invitrogen) for 2 h. No antibody amplification was necessary for the tTA::miR-212 dsRED signal. Sections were also incubated for 10 min with the nuclear stain DRAQ5 (1:10,000 dilution; BioStatus Limited, UK and Hoechst 33258 (1:1000 dilution: Sigma), followed by an additional three 10-min washes. Sections were mounted on slides with Fluoromount-G (SouthernBiotech) and images captured using a 16-bit digital camera (Micromax YHS 1300; Princeton Instruments) mounted on an inverted Leica microscope (DM IRB).
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