For pendrin-expression analysis, tissue sections were harvested from the inner ears of Slc26a4919-2A>G/C565Y and Slc26a4C565Y/C565Y mice. Tissue sections were mounted onto silane-coated glass slides, deparaffinized in xylene, and rehydrated in ethanol. Tissue samples were stained with rabbit antipendrin (1:1000; antibody kindly provided by Dr. Jinsei Jung, Yonsei University College of Medicine, Seoul, Korea), and incubated with DAPI (1:5000) and Alexa Fluor 488-conjugated goat antirabbit IgG (H + L) secondary antibodies (1:200; Thermo Fisher). After incubation, slides were washed with PBS and mounted with the ProLong Antifade Kit at 25 °C. Images were captured using a laser-scanning confocal microscope (LSM880, Zeiss).
Alexa fluor 488 conjugated goat antirabbit igg h l secondary antibodies
Manufactured by Thermo Fisher Scientific
Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibodies are fluorescently labeled antibodies that bind to rabbit primary antibodies. They are used for detection and visualization in various immunoassay techniques.
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2 protocols using alexa fluor 488 conjugated goat antirabbit igg h l secondary antibodies
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Pendrin Expression Analysis in Mouse Inner Ear
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Pendrin Expression Analysis in Mouse Inner Ear
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For pendrin expression analysis, tissue sections were harvested from the inner ears of Slc26a4 919-2A>G/C565Y and Slc26a4 C565Y/C565Y mice. Tissue sections were mounted onto the silane-coated glass slides and then were deparaffinized in xylene and rehydrated in ethanol. Tissue were stained with rabbit anti-pendrin, (1:1000; antibody was kindly provided by Dr. Jinsei Jung, Yonsei University College of Medicine, Seoul, Republic of Korea), then incubated with DAPI (1:5000) and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibodies (1:200; Thermo Fisher). After incubation, the slides were washed with PBS and mounted with the ProLong Antifade Kit at 25 °C.
Images were captured using a laser scanning confocal microscope (LSM880, Zeiss).
Images were captured using a laser scanning confocal microscope (LSM880, Zeiss).
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