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Cell proliferation elisa

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The Cell Proliferation ELISA is a laboratory equipment product that measures cell proliferation using an enzyme-linked immunosorbent assay (ELISA) technique. It provides quantitative assessment of cell growth and division.

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Lab products found in correlation

Synergy h1, by Agilent Technologies (1 mentions) Z2 coulter particle count and size analyzer, by Beckman Coulter (1 mentions) Anti cd3 cd28 coated dynabeads, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

BrdU cell proliferation enzyme-linked immunosorbent assay (ELISA) kit Cell Proliferation ELISA 5-bromo-2′-deoxyuridine (BrdU) colorimetric kit Cell Proliferation ELISA BrdU kit Cell Proliferation ELISA BrdU Cell Proliferation ELISA Kit ELISAs

4 protocols using cell proliferation elisa

1

Cell Proliferation Assay Protocol

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Cells were seeded in 96-well plate at 5000 cells/well density. After 24 h of incubation, cellular growth was assessed by bromodeoxyuridine (BrdU) incorporation assay by using cell proliferation ELISA (Sigma-Aldrich) according to the manufacture’s protocol.
Hamada S., Matsumoto R., Tanaka Y., Taguchi K., Yamamoto M, & Masamune A. (2021). Nrf2 Activation Sensitizes K-Ras Mutant Pancreatic Cancer Cells to Glutaminase Inhibition. International Journal of Molecular Sciences, 22(4), 1870.
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2

Cell Proliferation Measurement Assay

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100–500 cells per well were plated in clear 96-well plates, in 100 μL RPMI media with 10% FBS. Cells were treated with the indicated drug concentrations for 5 days and cell proliferation was measured using the Cell Proliferation ELISA, BrdU (colorimetric; Sigma-Aldrich) assay and Synergy H1 plate reader (BioTek) following manufacturer’s protocol.
Nassar K.W., Hintzsche J.D., Bagby S.M., Espinoza V., Langouët-Astrié C., Amato C.M., Chimed T.S., Fujita M., Robinson W., Tan A.C, & Schweppe R.E. (2021). Targeting CDK4/6 Represents a Therapeutic Vulnerability in Acquired BRAF/MEK Inhibitor–Resistant Melanoma. Molecular cancer therapeutics, 20(10), 2049-2060.
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3

Cell Proliferation ELISA Protocol

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Following termination of compression, proliferation was evaluated using a Cell Proliferation ELISA (Sigma-Aldrich) according to the manufacturer's specifications. Alternatively, MCAs were dispersed by incubation in 1 ml 0.25% trypsin for 10 min. Suspensions were diluted in 9 ml PBS, then counted using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter, Brea, CA, USA). Assays were performed in triplicate and P-values were calculated by paired Student's t-test comparing compressed versus uncompressed cells.
Klymenko Y., Wates R.B., Weiss-Bilka H., Lombard R., Liu Y., Campbell L., Kim O., Wagner D., Ravosa M.J, & Stack M.S. (2018). Modeling the effect of ascites-induced compression on ovarian cancer multicellular aggregates. Disease Models & Mechanisms, 11(9), dmm034199.
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4

Proliferation Assay of PBMCs Treated with FL118

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PBMCs from healthy donors were seeded in 96-well flat-bottom plates at 4 Â 10 4 cells/well and stimulated with anti-CD3/CD28coated dynabeads (Life Technologies) in a bead-to-cell ratio of 1:3 and 50 U/mL recombinant human IL2. After 24 hours, the PBMCs were treated with serial dilutions of FL118 or a DMSO control, representing the highest DMSO content, for 24 hours. BrdUrd was added 16 hours prior to the termination of proliferation. BrdUrd incorporation was measured by Cell Proliferation ELISA (Sigma-Aldrich) according to the manufacturer's protocol.
Holthof L.C., van der Schans J.J., Katsarou A., Poels R., Gelderloos A.T., Drent E., van Hal-van Veen S.E., Li F., Zweegman S., van de Donk N.W.C.J., Themeli M., Groen R.W.J, & Mutis T. (2021). Bone Marrow Mesenchymal Stromal Cells Can Render Multiple Myeloma Cells Resistant to Cytotoxic Machinery of CAR T Cells through Inhibition of Apoptosis. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(13).
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