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Legendplex bead

Manufactured by BioLegend
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LegendPlex beads are a set of magnetic beads designed for multiplex detection of analytes in biological samples. The beads are coated with specific antibodies that can capture and detect multiple target molecules simultaneously.

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Lab products found in correlation

Peroxidase conjugated streptavidin, by Jackson ImmunoResearch (2 mentions) Mouse th cytokine panel 13 plex, by BioLegend (2 mentions) Purified ifn γ clone r4 6a2, by Thermo Fisher Scientific (2 mentions) Biotin ifn γ clone xmg1, by Thermo Fisher Scientific (2 mentions) Cpg a, by InvivoGen (1 mentions) Poly 1 c hmw, by InvivoGen (1 mentions) Poly 1 c lmw, by InvivoGen (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Cpg b, by InvivoGen (1 mentions) Flt3l, by Thermo Fisher Scientific (1 mentions) Imiquimod imq, by InvivoGen (1 mentions) Gm csf, by R&D Systems (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions)

Close spelling variants of this product

LEGENDplex™ bead-based multiplex assays LegendPlex™ cytometric Bead array LEGENDplex bead-based immunoassay KIT LEGENDplex bead array LEGENDplex cytokine bead array Human T Helper Cytokine Panel LEGENDplex bead-based immunoassay LEGENDplex multiplex bead assay LegendPlex cytometric bead assays LegendPlex TM Multiplex cytokine bead-based assay LEGENDplex bead-based immunoassay

5 protocols using legendplex bead

1

Serum Cytokine Profiling in MCMV

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Serum cytokines from time-course experiments were measured by LEGENDPlex bead-based immunoassay using the Mouse Th Cytokine Panel (13-plex), per the manufacturer’s instructions (Biolegend). Serum IFN-γ levels on day 7 post-MCMV were determined via sandwich ELISA using the following antibodies: purified IFN-γ (clone R4–6A2, eBioscience), Biotin-IFN-γ (clone XMG1.2, eBioscience), and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Labs) (Wesley et al., 2005 (link)).
Anderson C.K., Reilly E.C., Lee A.Y, & Brossay L. (2019). Qa-1-Restricted CD8+ T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection. Cell reports, 27(2), 537-548.e5.
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2

Serum Cytokine Profiling in MCMV

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Serum cytokines from time-course experiments were measured by LEGENDPlex bead-based immunoassay using the Mouse Th Cytokine Panel (13-plex), per the manufacturer’s instructions (Biolegend). Serum IFN-γ levels on day 7 post-MCMV were determined via sandwich ELISA using the following antibodies: purified IFN-γ (clone R4–6A2, eBioscience), Biotin-IFN-γ (clone XMG1.2, eBioscience), and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Labs) (Wesley et al., 2005 (link)).
Anderson C.K., Reilly E.C., Lee A.Y, & Brossay L. (2019). Qa-1-Restricted CD8+ T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection. Cell reports, 27(2), 537-548.e5.
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3

Generating Dendritic Cells from Bone Marrow

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Bone marrow from tibiae and femurs was flushed with PBS and subjected to erythrocyte lysis. Cells were adjusted to 106 cells/ml in fully supplemented RPMI-1640. Medium was supplemented with 100ng/ml Flt3L (eBioscience) to generate FL-DCs or 20ng/ml GM-CSF (R&D) and 5ng/ml IL-4 (Peprotech) to generate G4-DCs. Fresh medium (50% of initial volume) supplemented with Flt3L, or GM-CSF/IL-4 was added after 3 days. Non-adherent cells in GM-CSF/IL-4 culture or total Flt3L cultured cells were harvested on day 7 and 106 cells were adoptively transferred i.v. into C57BL/6J recipients. FL-DCs were further stimulated for 24 h with poly(I:C)-HMW (1µg/ml, Invivogen), poly(I:C)-LMW (1µg/ml, Invivogen), LPS (10ng/ml; Invivogen), imiquimod (IMQ, 1µg/ml, Invivogen), CpG-ODN1585 (CpG-A, 1µM, Invivogen), CpG-ODN1668 (CpG-B, 1µM, Invivogen) and supernatants were analyzed for IL-6 protein using flow cytometry (Legendplex beads, Biolegend) or ELISA (R&D).
Kuttke M., Hromadová D., Yildirim C., Brunner J.S., Vogel A., Paar H., Peters S., Weber M., Hofmann M., Kerndl M., Kieler M., Datler H., Musiejovsky L., Salzmann M., Lang M., Soukup K., Halfmann A., Sharif O, & Schabbauer G. (2022). PI3K Signaling in Dendritic Cells Aggravates DSS-Induced Colitis. Frontiers in Immunology, 13, 695576.
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4

Analyzing iNKT cell responses to α-GalCer

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Mice were injected with OCH (an α-GalCer analogue, 5 μg/mouse) i.g. or i.v. and were euthanized 24h later. Cytokine-producing iNKT cells in the intestine and other organs were examined by flow cytometry as described above. Blood plasma was examined for the levels of IL-2, IL-4, and IFN-γ by Legendplex beads (BioLegend).
Liu Q, & Kim C.H. (2019). Control of tissue-resident iNKT cells by vitamin A metabolites and P2X7-mediated cell death. Journal of immunology (Baltimore, Md. : 1950), 203(5), 1189-1197.
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5

Multiplex Cytokine Profiling of Cell Cultures

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The detection of secreted cytokines in culture medium was performed using BioLegend LEGENDplex beads according to the manufacturer’s directions. Briefly, culture medium from cells was centrifuged to remove contaminating cells and debris, and immediately stored at −80 °C until used. Samples and known standards for each cytokine analyzed were subsequently incubated with cytokine-capture beads and biotinylated-detection antibodies in polypropylene microfuge tubes shaking at 1000 rpm at room temperature for 2 hours. Following this incubation, SA-PE was added, followed by a 30 min incubation while shaking at 1000 rpm. Beads were washed and immediately analyzed via flow cytometry.
LEGENDplex beads were analyzed on an LSRII (BD Bio-sciences). Standard curves were generated and data were analyzed with LEGENDplex software (Biolegend/ VigeneTech). Cytokine levels in samples were confirmed using BioLegend LEGEND MAX sandwich cytokine-capture ELISA kits according to manufacturer’s protocol.
Reed J, & Wetzel S. (2019). Trogocytosis-mediated Intracellular Signaling in CD4+ T cells Drives TH2-associated Effector Cytokine Production and Differentiation,. Journal of immunology (Baltimore, Md. : 1950), 202(10), 2873-2887.
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