Protein samples from cultured HEK-293T cells were separated using sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride filters. The filters were incubated overnight at 4 °C with appropriate antibodies. Secondary antibodies conjugated to horseradish peroxidase were added to the filters and then visualized in the enhanced chemiluminescence solution (Thermo Scientific, USA). The visualization was performed via the ImageQuant LAS 4000 mini Molecular Imaging System (GE Healthcare Life Sciences, USA), and the Image J software (NIH, USA) was used for the analysis of band intensity. The antibodies used were as follows: β-actin (1:1000; Chemicon, Cat # MAB1501), FLAG (1:4000; Sigma-Aldrich/Merck Millipore, Cat # F1804), GABAAR-α5 (1: 1000; Chemicon, Cat # AB9678), and GABAAR-γ2 (1:100; Santa Cruz, Cat # sc-101963).
Imagequant las 4000 mini molecular imaging system
Manufactured by GE Healthcare
Sourced in United States
The ImageQuant LAS 4000 mini Molecular Imaging System is a compact, high-performance imaging device designed for life science research applications. It utilizes a charge-coupled device (CCD) camera and multiple excitation light sources to capture and analyze images of various molecular samples, such as fluorescent proteins, chemiluminescent blots, and radioisotope-labeled samples.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Merck Group (2 mentions)
Enhanced chemiluminescence solution, by Thermo Fisher Scientific (1 mentions)
Gabaar γ2, by Santa Cruz Biotechnology (1 mentions)
Flag tag (flag), by Merck Group (1 mentions)
Asic1a, by Santa Cruz Biotechnology (1 mentions)
Glua1, by Abcam (1 mentions)
Psd95, by Abcam (1 mentions)
Glua2, by Abcam (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
Glun2a, by Merck Group (1 mentions)
Glun2b, by Merck Group (1 mentions)
3 protocols using imagequant las 4000 mini molecular imaging system
1
Western Blot Analysis of GABA Receptors
2
Western Blot Analysis of Protein Samples
3
Quantifying Brain Region-Specific Protein Profiles
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Protein samples from different regions of mouse brain, including insular cortex and other areas, were separated by SDS–polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride filters. The filters were incubated overnight at 4 °C with appropriate antibodies. Secondary antibodies conjugated to horseradish peroxidase were added to the filters and then visualized in ECL solution. The visualization was performed via the ImageQuant LAS 4000 mini Molecular Imaging System (GE Healthcare Life Sciences), and the Image J software (NIH) was used for the analysis of band intensity. Antibodies used were as follows: ASIC1a (1:500; Santa Cruz, sc-13905), β-actin (1:1,000; Chemicon, Cat # MAB1501), GAPDH (1:1,000; KangChen, Cat # KC-5G4), GluA1 (1:1,000; Epitomics, Cat # 3861-1), GluA2 (1:1,000; Epitomics, Cat # 3520-1), GluN1 (1:1,000; R&D Systems, Cat # PPS011B), GluN2A (1:1,000; Millipore, Cat # 07-632), GluN2B (1:1,000; Millipore, Cat # MAB5220), PSD-95 (1:1,000; Epitomics, Cat # 2366-1), pGSK3β-Ser9/pGSK3α-Ser21 (1:1,000; Cell Signaling Technology, Cat # 8566) and GSK3β (1:1,000; Cell Signaling Technology, Cat # 12456).
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