Log In Sign up
The largest database of trusted experimental protocols

Akt ph gfp

Manufactured by Addgene
Visit Supplier

AKT-PH-GFP is a fluorescent protein construct that contains the pleckstrin homology (PH) domain of the AKT protein fused to green fluorescent protein (GFP). This construct can be used to visualize the localization of AKT in live cells.

Automatically generated - may contain errors

Lab products found in correlation

Ph btk gfp, by Addgene (2 mentions) Cf inp d281a, by Addgene (1 mentions) Gfp plcδ ph, by Addgene (1 mentions) Gfp p4m sidm, by Addgene (1 mentions) Gfp p4m sidmx2, by Addgene (1 mentions) Pbgpa cmv gfp osbp ph domain, by Addgene (1 mentions) Mkate2 p2a apex2 tapp1 ph, by Addgene (1 mentions) Ph plcd1 gfp, by Addgene (1 mentions) Ph plcd1 r40l gfp, by Addgene (1 mentions) Gfp drd2, by Addgene (1 mentions) E cadherin gfp, by Addgene (1 mentions) Ezrin gfp, by Addgene (1 mentions) Amaxa nucleofector, by Lonza (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

5 protocols using akt ph gfp

1

Protein Labeling and Imaging Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mutations were introduced into DOC2B-GFP N2 7 (link), C2AB-GFP N2 or C2B-GFP N2 6 (link), using site-directed mutagenesis PCR. Botulinum toxins BoNT/C and BoNT/E were a generous gift from the laboratory of Prof. Ilana Lotan (Tel-Aviv University). mRFP-syx1A and SNAP25-YFP were a kind gift from Prof. Edward Stuenkel (University of Michigan). R-GECO was a generous gift from the laboratory of Prof. Robert Campbell 31 (link). Lact-C2-GFP was a gift from Prof. Sergio Grinstein (addgene #22852). For PI manipulations using the rapamycin dimerization system, the PH domain of PLCδ1, PLCδ-PH-GFP (addgene #21179), CF-Inp (addgene #20155), CF-InpD281A (addgene #20156) and Lyn11-FRB (addgene #20147) were a gift from Prof. Tobias Meyer. CF-PIPK, CF-PIPKi, mcherry-iSH were a generous gift from the laboratory of Prof. Takanari Inoue (Johns Hopkins University). PLCδ-PH-mKate was a kind gift from Prof. Thomas Martin (University of Wisconsin-Madison). Rab7-FRB (addgene #51613), PH-Akt-GFP (addgene #51465), and PH-Btk-GFP (addgene #51463) were a gift from Prof. Tamas Balla. NPY-mRFP was a kind gift from Prof. Matthijs Verhage (Vrije University).
Michaeli L., Gottfried I., Bykhovskaia M, & Ashery U. (2017). Phosphatidylinositol (4,5)-bisphosphate targets DOC2B to the plasma membrane. Traffic (Copenhagen, Denmark), 18(12), 825-839.
+ Open protocol
+ Expand
2

Plasmid Transfection and Infection in RAW264.7 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following probes GFP-P4M-SidM (Addgene plasmid # 51469); GFP-P4M-SidMx2(Addgene plasmid #51472); PH-Akt-GFP (Addgene plasmid # 51465); PH-Akt(R25C)-GFP (Addgene plasmid # 51466) PH-PLCD1-GFP (Addgene plasmid # 51407); PH-PLCD1(R40L)-GFP (Addgene plasmid # 51408); PH-Btk-GFP (Addgene plasmid # 51463); PH-Btk(R28C)-GFP (Addgene plasmid # 51464) were gifts from Tamas Balla; GFP-EEA1 wt (Addgene plasmid # 42307) was a gift from Silvia Corvera; pBGPa-CMV-GFP-OSBP PH domain (Addgene plasmid # 58840) was a gift from Tim Levine & Sean Munro. mKate2-P2A-APEX2-TAPP1-PH (Addgene Plasmids #67662) was a gift was a gift from Rob Parton. Plasmids were isolated and test digested for confirmation. They were then introduced into RAW264.7 by nucleofection using reagents from Mirus Bio (Madison, Wisconsin). After seeding transfected cells and overnight culture, they were infected. Infection of transfectants were evaluated after 2 or 24hr.
Zhang N., Prasad S., Despointes C.E., Young J, & Kima P.E. (2018). Leishmania parasitophorous vacuole membranes display phosphoinositides that create conditions for continuous Akt activation and a target for miltefosine in Leishmania infections. Cellular microbiology, 20(11), e12889.
+ Open protocol
+ Expand
3

Visualizing Cellular Dynamics in Plasmodium Infection

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary mouse hepatocytes were isolated and cultivated as described previously (29 (link)). HeLa cells (gift from Robert Menard, Pasteur Institute, Paris, France) were authenticated by short tandem repeat DNA profiling (Microsynth) and cultured as described before (30 (link)). We transfected 2 × 106 HeLa cells with 4 µg of plasmid DNA of the following fluorescent reporter constructs with program T-28 in an Amaxa Nucleofector (Lonza): mCherry-utrophin (7 (link)) (Addgene plasmid 26740), GFP-utrophin (7 (link)) (Addgene plasmid 26737), GFP-C1-PLCdelta-PH (12 (link)) (Addgene plasmid 21179), AKT-PH-GFP (13 (link)) (Addgene plasmid 18836), Lact-C2-GFP (14 (link)) (Addgene plasmid 22853), ezrin-GFP (15 (link)), ezrin(T567D)-GFP (16 (link)) (Addgene plasmid 20681), E-cadherin–GFP (17 (link)) (Addgene plasmid 28009), A2BR-YFP (18 (link)) (Addgene plasmid 37202), GFP-DRD2 (19 (link)) (Addgene plasmid 24099), PM-GFP (20 (link)) (Addgene plasmid 21213), and GFP-GPI (TRAIL and DAF) (21 (link)). Cells were subsequently used to seed glass bottom dishes (MatTek, In Vitro Scientific) and infected the next day with P. berghei sporozoites as previously described (28 (link)).
Burda P.C., Caldelari R, & Heussler V.T. (2017). Manipulation of the Host Cell Membrane during Plasmodium Liver Stage Egress. mBio, 8(2), e00139-17.
+ Open protocol
+ Expand
4

Plasmid Transfection Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
AKT-PH-GFP and GFP-PDK1 plasmids were from Addgene. pMX-myc, pMX-wIPMK and pMX-IPMK-KSA were generated in-lab.
Reilly L., Semenza E.R., Koshkaryan G., Mishra S., Chatterjee S., Abramson E., Mishra P., Sei Y., Wank S.A., Donowitz M., Snyder S.H, & Guha P. (2023). Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis. iScience, 26(5), 106623.
+ Open protocol
+ Expand
5

Transfection of AKT-PH-GFP in MiaPaCa-2 cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
MiaPaCa-2 cells were transfected with the plasmid containing a cDNA encoding a green fluorescent protein (GFP) tagged-AKT pleckstrin homology domain (AKT-PH-GFP) from Addgene (pcDNA3-AKT-PH-GFP cat # 18836) by using Lipofectamine 2000 (Invitrogen) as suggested by the manufacturer. Analysis of the cells transiently transfected was performed 24 h after transfection.
Soares H.P., Ming M., Mellon M., Young S.H., Han L., Sinnet-Smith J, & Rozengurt E. (2015). Dual PI3K/mTOR inhibitors induce rapid over-activation of the MEK/ERK pathway in human pancreatic cancer cells through suppression of mTORC2. Molecular cancer therapeutics, 14(4), 1014-1023.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!