P5655

Manufactured by Merck Group

Sourced in Germany

P5655 is a laboratory equipment product manufactured by Merck Group. It is a general-purpose device designed for use in various scientific research and testing applications. The core function of P5655 is to provide a controlled environment for conducting experiments and analyses. No further details about the intended use or specific features of this product can be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

6 protocols using p5655

Phytic Acid and Phosphate Determination

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Samples were extracted in duplicate according to the following procedure: about 100 mg sample (flours and freeze-dried purées) was suspended in 5 ml of 0.5 M HCl and boiled for 6 min. After drying, the extract was solubilized in milliQ water and injected for phytic acid (IP6) and phosphate content determination using high-performance anion-exchange chromatography (Dionex, Sunnyvale, CA, USA) according to the method described by Baye et al. (2013) (link). Inositol-6-phosphate (IP6) from rice (Sigma, P3168) and KH₂PO₄ (Sigma, P5655) were used as standards. Results were averaged and expressed in mg/100g on dry matter basis.

Summo C., De Angelis D., Rochette I., Mouquet-Rivier C, & Pasqualone A. (2019). Influence of the preparation process on the chemical composition and nutritional value of canned purée of kabuli and Apulian black chickpeas. Heliyon, 5(3), e01361.

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Bacillus subtilis Growth Media Comparison

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Parallel cultures of B. subtilis (lab strain) were cultured in rich or mineral media in baffled flasks. Flasks were incubated at 37 °C with continuous stirring at 200 rpm. The rich media was prepared from 10 g/L tryptone (T9410, Sigma-Aldrich), 10 g/L NaCl (27810.295, VWR) and 5 g/L yeast extract (92144, Sigma-Aldrich). The mineral media was prepared in MilliQ-H₂O (MQ-H₂O) by dissolving 11.2 g/L Na₂HPO₄-7H₂O (S9390, Sigma-Aldrich), 3 g/L KH₂PO₄ (P5655, Sigma-Aldrich), 0.5 g/L NaCl (27810.295, VWR), 0.5 g/L NH₄Cl (A9434, Sigma-Aldrich), 0.2465 g/L MgSO₄-7H₂O (M5921, Sigma-Aldrich), 0.1470 g/L CaCl2-2H₂O (223506, Sigma-Aldrich), 4 g/L glucose (101176K, VWR) and 1 mL/L media of a trace element solution containing 10 g/L FeSO₄-7H₂O (F8633, Sigma-Aldrich), 2.25 g/L ZnSO₄-7H₂O (Z0251, Sigma-Aldrich), 2 g/L CaCl₂-2H₂O (223506, Sigma-Aldrich), 1 g/L CuSO₄-5H₂O (197722500, Thermo Fisher Scientific), 0.38 g/L MnCl₂-4H₂O (M5005, Sigma-Aldrich), 0.14 g/L H₂BO₃ (B6768, Sigma-Aldrich), and 0.1 g/L (NH₄)₆Mo₇O₂₄-4H₂O (1011820250, Merck Millipore, Damstadt, Germany). The final media was supplemented with 600 µg/L CoCl₂-6H₂O (33606, VWR), 1 mg/L biotin (47868, Sigma-Aldrich), and 1 mg/L thiamine hydrochloride (T1270, Sigma-Aldrich).

Røst L.M., Brekke Thorfinnsdottir L., Kumar K., Fuchino K., Eide Langørgen I., Bartosova Z., Kristiansen K.A, & Bruheim P. (2020). Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems. Metabolites, 10(2), 74.

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Preparation of Defined Microbial Growth Medium

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To milli-Q water (1 L) were added KH₂PO₄ (13.60 g, Sigma-Aldrich, Cat.#P5655), (NH₄)₂SO₄ (2.00 g, Sigma-Aldrich Cat.# A4418) and glucose (2.00 g, Sigma-Aldrich Cat.#G7021), and the pH of the solution was adjusted to 7.00 ± 0.09 with KOH. The medium was then autoclaved at 121 °C with a 30 min sterilization cycle. After the solution cooled down to room temperature, syringe filter-sterilized (0.22 μm nitrocellulose syringe filters) solutions of MgSO₄ (1 mL, 0.2 g mL⁻¹ of MgSO₄·7H₂O, Sigma-Aldrich Cat. #M2773) and FeSO₄ (500 μL, 1.0 mg mL⁻¹ of FeSO₄·7H₂O, Sigma-Aldrich Cat.#F8633) were added to the medium broth. The medium was stored in the dark at room temperature until use.

Bordeleau E., Mazinani S.A., Nguyen D., Betancourt F, & Yan H. (2018). Abrasive treatment of microtiter plates improves the reproducibility of bacterial biofilm assays. RSC Advances, 8(57), 32434-32439.

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Glucose Mineral Medium for Shake Flasks

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The glucose mineral medium for shake flasks was prepared in milliQ-H₂O (18.2 MΩ cm) by dissolving 11.2 g/l Na₂HPO₄–7H₂O (S9390, Sigma-Aldrich), 3 g/l KH₂PO₄ (P5655, Sigma-Aldrich), 0.5 g/l NaCl (27810.295, VWR), 1 g/l NH₄Cl (A9434, Sigma-Aldrich), 0.2465 g/l MgSO₄–7H₂O (M5921, Sigma-Aldrich), 4 g/l glucose (101176 K, VWR), 2 ml/l of a 50 mg/l CoCl₂–6H₂O solution (C8661, Sigma-Aldrich) and 2 ml/l medium of a trace element solution containing 10 g/l FeSO₄–7H₂O (F8633, Sigma-Aldrich), 2.25 g/l ZnSO₄–7H₂O (Z0251, Sigma-Aldrich), 2 g/l CaCl₂–2H₂O (223506, Sigma-Aldrich), 1 g/l CuSO₄–5H₂O (197722500, Fisher Scientific), 0.38 g/l MnCl₂–4H₂O (M5005, Sigma-Aldrich), 0.14 g/l H₂BO₃ (B6768, Sigma-Aldrich) and 0.1 g/l (NH₄)6Mo₇O₂₄–4H₂O (1011820250, Merck).

Thorfinnsdottir L.B., Bø G.H., Booth J.A, & Bruheim P. (2023). Survival of Escherichia coli after high-antibiotic stress is dependent on both the pregrown physiological state and incubation conditions. Frontiers in Microbiology, 14, 1149978.

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Preparation of M9 Buffer for Microbial Cultivation

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To create the M9 buffer, 3.0 g of KH₂PO₄ (potassium phosphate; CASnr. 7778-77-0; e.g., Sigma, P5655), 7.5 g of Na₂HPO₄ * 2 H₂O (sodium phosphate dihydrate; CASnr. 10028-24-7; e.g., Sigma, 30412), and 5.0 g of NaCl (sodium chloride; CASnr. 7647-14-5; e.g., Sigma, S5886) were transferred into an autoclavable 1 L bottle and filled until 1000 mL with Milli-Q. The bottle was autoclaved for 20 min at 120 °C, and let cool to at least 55 °C. In a sterile environment, 1 mL of 1 M MgSO₄ (magnesium sulphate; CASnr. 7487-88-9; e.g., Sigma, M2643) was added and mixed well.

van Es-Remers M., Spadaro J.A., Poppelaars E., Kim H.K., van Haaster M., de Wit M., ILiopoulou E., Wildwater M, & Korthout H. (2022). C. elegans as a test system to study relevant compounds that contribute to the specific health-related effects of different cannabis varieties. Journal of Cannabis Research, 4, 53.

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Preparation of Mouse Cerebellar Slices

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Five- to seven-week-old C57BL/6J mice (The Jackson Laboratory, RRID:IMSR_JAX:000664) were terminally anesthetized in a CO₂ chamber and then transcardially perfused with 1× DPBS (Gibco 14190-250; Thermo Fisher Scientific, Waltham, MA, USA). The cerebellum was extracted and transferred into chilled artificial cerebrospinal fluid (124 mM NaCl; Macron 7581-12, 2.5 mM KCl; Sigma-Aldrich P3911, 2.0 mM MgSO₄; Sigma-Aldrich M3409, 1.25 mM KH₂PO₄; Sigma-Aldrich P5655, 26 mM NaHCO₃; Sigma-Aldrich 93350, 10 mM glucose; Macron 4912-12). The cerebellum was sectioned into 300 µm coronal slices using a vibratome Ci 7000 smz (Campden Instruments; Lafayette, IN, USA). Cerebellum slices were submerged in Minimum Essential Media (MEM; Gibco 11095; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 15% fetal bovine serum (FBS; Gibco 26140079; Themo Fisher Scientific, Waltham, MA, USA), 100 mg/mL penicillin G, and 100 mg/mL streptomycin (Gibco 15140122; Thermo Fisher Scientific, Waltham, MA, USA) until use [72 (link)].

Ampudia-Mesias E., Cameron C.S., Yoo E., Kelly M., Anderson S.M., Manning R., Abrahante Lloréns J.E., Moertel C.L., Yim H., Odde D.J., Saydam N, & Saydam O. (2024). The OTX2 Gene Induces Tumor Growth and Triggers Leptomeningeal Metastasis by Regulating the mTORC2 Signaling Pathway in Group 3 Medulloblastomas. International Journal of Molecular Sciences, 25(8), 4416.

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