Neutrophil depletion was achieved by intraperitoneal doses of 1A8 monoclonal anti-Ly6G antibody (Bio X Cell, Lebanon, NH) at 25 mg/kg in saline. Equivalent doses of IgG monoclonal anti-trinitrophenol (clone 2A3, Bio X Cell, Lebanon, NH) were administered to control mice 24 hours before carrying out the dorsal skin edema inflammation model. Four hours after dorsal skin model, mice were killed by anaesthetization, and whole blood was collected through cardiac puncture for flow cytometric analysis of neutrophils. The heparinized blood (1:100, stock heparin 5,000 IU/ml) was incubated with the following antibodies: anti-Ly6G-BV785 (1:200, catalog 127645, BioLegend, San Diego, CA) and anti-Ly6C BV605 (1:200, catalog 128035, BioLegend, San Diego, CA). Samples were acquired in an LSRFORTESSA flow cytometer (BD Biosciences, Oxford, United Kingdom) and analyzed using FlowJo software 9.7.5 (FlowJo, Ashland, OR).
Anti ly6g bv785
Manufactured by BioLegend
Anti-Ly6G-BV785 is a lab equipment product produced by BioLegend. It is a fluorescently-labeled antibody that targets the Ly6G antigen.
Automatically generated - may contain errors
2 protocols using anti ly6g bv785
1
Neutrophil Depletion and Skin Inflammation Analysis
2
Analyzing Nanoparticle Uptake in Lung Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The ALI mice were treated with Cy5.5-labeled NPs for 22 h. The cell suspensions in BALF were stained with anti-CD11b-AF488, anti-CD11c-APC, anti-F4/80-BV421, and anti-Ly6G-BV785 antibodies (BioLegend), and analyzed using CytoFLEX flow cytometer (Beckman Coulter, Inc.). Data were analyzed with FlowJo software X.
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