Antibodies used in this study included mouse monoclonals against β-actin (C4), PRODH (A-11), TOM20, and Rieske FeS IgG (A-5) from Santa Cruz Biotechnology (Santa Cruz, CA); HRP-conjugated goat anti-mouse secondary from Bio-Rad Laboratories, Inc. (Hercules, CA); α-tubulin mouse monoclonal (T9026) from MilliporeSigma (St. Louis, MO); YME1L1, GRP-75, and HSP-60 rabbit polyclonals from ProteinTech™ (Rosemont, IL); Alexa 488 goat anti-mouse and 594 goat anti-rabbit secondaries from Life Technologies (Thermo Fisher Scientific, Carlsbad, CA).
Alexa 488 goat anti mouse and 594 goat anti rabbit secondaries
Alexa Fluor 488 goat anti-mouse and 594 goat anti-rabbit secondaries are fluorescently-labeled antibodies used in immunofluorescence assays. These secondary antibodies bind to the primary antibodies that target specific proteins or cellular structures, allowing for the visualization and detection of these targets.
Lab products found in correlation
2 protocols using alexa 488 goat anti mouse and 594 goat anti rabbit secondaries
Antibody Validation for Mitochondrial Proteins
Antibodies used in this study included mouse monoclonals against β-actin (C4), PRODH (A-11), TOM20, and Rieske FeS IgG (A-5) from Santa Cruz Biotechnology (Santa Cruz, CA); HRP-conjugated goat anti-mouse secondary from Bio-Rad Laboratories, Inc. (Hercules, CA); α-tubulin mouse monoclonal (T9026) from MilliporeSigma (St. Louis, MO); YME1L1, GRP-75, and HSP-60 rabbit polyclonals from ProteinTech™ (Rosemont, IL); Alexa 488 goat anti-mouse and 594 goat anti-rabbit secondaries from Life Technologies (Thermo Fisher Scientific, Carlsbad, CA).
Antibody Validation for Mitochondrial Proteins
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