A549 cells (1 × 104 cells/well) were grown in a 10 × 35 mm cell culture dish (Greiner Bio-One, Frickenhausen, Germany) for 24 h. After treatment of 10 µM BODIPY-PF-543 for 30 min cells were fixed for 10 min with 4% formaldehyde, following treatment of 10 µM Diamidine-2-phenylindole dihydrochloride (DAPI) dye for 20 min. The images were captured using ZEISS LSM710 confocal microscope. Images were analyzed using ZEN blue 2.3 lite software (Carl Zeiss Microscopy GmbH, Jena, Germany) and fluorescence intensity of the treated group was compared with non-treated cells.
Zen blue 2.3 lite software
Manufactured by Zeiss
Sourced in Germany
ZEN blue 2.3 lite software is a microscope imaging and analysis software from Zeiss. The software provides basic image acquisition and analysis functionalities for Zeiss microscope systems.
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Lab products found in correlation
3 protocols using zen blue 2.3 lite software
1
Cellular Localization of BODIPY-PF-543
2
Confocal Imaging of Transgenic Roots
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Immunostained root sections were imaged on a confocal microscope (Zeiss LSM 780).
Autofluorescence observation was performed using an argon laser at 405 nm and secondary antibodies were excited at 561 nm. Both were detected at a 566-679 nm window using the same settings (gain, offset and resolution which were manually set up) to allow quantification measurements (detailed in the Supplemental Methods S1).
For LaPG3::nlsYFP, DR5::nlsYFP and screening of transformed cluster roots, mCherry internal control and nlsYFP were excited at 561 nm and 514 nm respectively and detected at 583-696 nm and 519-583 nm windows, respectively. Observations were made using Plan-Apochromat 10x/0.45 M27 and 20x/0.8 M27 objectives. Image acquisition was performed with the Zeiss ZEN black 2010 software and image analysis was conducted using the ZEN blue 2.3 lite software (Carl Zeiss Microscopy).
Autofluorescence observation was performed using an argon laser at 405 nm and secondary antibodies were excited at 561 nm. Both were detected at a 566-679 nm window using the same settings (gain, offset and resolution which were manually set up) to allow quantification measurements (detailed in the Supplemental Methods S1).
For LaPG3::nlsYFP, DR5::nlsYFP and screening of transformed cluster roots, mCherry internal control and nlsYFP were excited at 561 nm and 514 nm respectively and detected at 583-696 nm and 519-583 nm windows, respectively. Observations were made using Plan-Apochromat 10x/0.45 M27 and 20x/0.8 M27 objectives. Image acquisition was performed with the Zeiss ZEN black 2010 software and image analysis was conducted using the ZEN blue 2.3 lite software (Carl Zeiss Microscopy).
3
Confocal Imaging of Transformed Plant Roots
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Immunostained root sections were imaged on a confocal microscope (Zeiss LSM 780). Autofluorescence observation was performed using an argon laser at 405 nm and secondary antibodies were excited at 561 nm. Both were detected at a 566-679 nm window using the same settings (gain, offset, resolution) to allow quantification measurements. For LaPG3::nlsYFP, DR5::nlsYFP and screening of transformed cluster roots, mCherry internal control and nlsYFP were excited at 561 nm and 514 nm respectively and detected at 583-696 nm and 519-583 nm windows, respectively. Observations were made using Plan-Apochromat 10x/0.45 M27 and 20x/0.8 M27 objectives. Image acquisition was performed with the Zeiss ZEN black 2010 software and image analysis was conducted using the ZEN blue 2.3 lite software (Carl Zeiss Microscopy).
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