The SKOV3 (serous carcinoma) and TOV-21G (clear cell carcinoma) human ovarian carcinoma cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). All cell lines were maintained in Dulbecco's modified Eagle's medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin. All cells were seeded into Cellstars® tissue culture plates (Greiner Bio-One GmbH, Frickenhausen, Germany) in a humidified incubator containing 5% CO2 at 37°C.
Cellstars tissue culture plates
Manufactured by Greiner
Sourced in Germany
Cellstars® tissue culture plates are a line of high-quality, sterile laboratory products designed for cell culture applications. These plates provide a controlled and consistent environment for the growth and maintenance of various cell types.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Tov 21g, by American Type Culture Collection (1 mentions)
Sb203580, by Merck Group (1 mentions)
Penicillin, by Nacalai Tesque (1 mentions)
Streptomycin, by Nacalai Tesque (1 mentions)
Gsk1120212, by Merck Group (1 mentions)
Rpmi 1640, by Nacalai Tesque (1 mentions)
2 protocols using cellstars tissue culture plates
1
Culturing Ovarian Cancer Cell Lines
2
Cultivation of Human Ovarian Cell Lines
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We maintained human serous ovarian cancer cell lines including OVCA420, OVCA433, OVCA429, TYK-nu, SKOV8, CAOV3, DOV13, HEYA8, A2780 and SKOV3 [8 (link)], in RPMI 1640 (Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (FBS) and 100 IU/ml penicillin and 100 μg/ml streptomycin (Nacali Tesque). The immortalized human ovarian surface epithelial cell line, HOSE-E7 [20 (link)], kindly provided by Dr. Katabuchi at Kumamoto University, was cultured in DMEM/F12 (Thermo Fisher Scientific, Waltham, USA) supplemented with 10% FBS and 100 IU/ml penicillin and 100 μg/ml streptomycin (Nacalai Tesque). 293FT cells were purchased from Thermo Fisher Scientific, and cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS and 100 IU/ml penicillin and 100 μg/ml streptomycin. All cells were seeded into Cellstars® tissue culture plates (Greiner, Frickenhausen, Germany) in a humidified incubator containing 5% CO2 at 37°C. All cell lines were regularly tested for mycoplasma.
As an inhibitor of the p38 MAPK pathway, we used SB203580 (Sigma-Aldrich, St.Louis, USA). To inhibit the ERK1/2 pathway, we used GSK1120212 (Sigma-Aldrich).
As an inhibitor of the p38 MAPK pathway, we used SB203580 (Sigma-Aldrich, St.Louis, USA). To inhibit the ERK1/2 pathway, we used GSK1120212 (Sigma-Aldrich).
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