6-week-old BALB/cJRj mice (Charles River) were i.p. infected with 5 x 105 parasites and sacrificed on days 2, 4, and 7 p.i. Immediately after being killed, mice were peritoneally lavaged with PBS, and recovered lavage fluid was centrifuged at 500 g for 8 min. Peritoneal cells were washed once with PBS. Harvested cells were suspended in stain buffer (PBS containing 2% FBS and 0.1 mM EDTA), seeded in 96-well plates (106 cells/100 μl), and pretreated with FcBlock (clone 2.4G2; BD Biosciences) for 30 min at 4°C. Cells were then incubated with fluorescently conjugated antibodies for cell surface markers from BD Biosciences: PE-conjugated anti-CD11b, PE-conjugated anti-Gr1 (clone RB6-8C5), PE-conjugated anti-F4/80, and APC-conjugated anti-Ly6G. Isotype controls consisted of PE-conjugated rat IgG2b, PE-conjugated rat IgG2a, and APC-conjugated rat IgG2a, provided by BD Biosciences. Analysis of stained cells was performed with a FACSCalibur flow cytometer (BD Biosciences). For all samples, 100,000 cells were analyzed for plot generation.
Pe conjugated rat igg2b
Manufactured by BD
PE-conjugated rat IgG2b is a fluorescently labeled antibody reagent. It is a type of immunoglobulin G (IgG) isotype derived from rats. The PE (phycoerythrin) fluorescent label is conjugated to the antibody, allowing for detection and analysis of target cells or molecules in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Pe conjugated anti cd11b, by BD (3 mentions)
Apc conjugated rat igg2a, by BD (3 mentions)
Apc conjugated anti ly6g, by BD (3 mentions)
Balb cjrj mice, by Charles River Laboratories (3 mentions)
Facscalibur flow cytometer, by BD (3 mentions)
Fc block clone 2.4g2, by BD (2 mentions)
Pe conjugated rat igg2a, by BD (2 mentions)
Pe conjugated anti gr1 clone rb6 8c5, by BD (2 mentions)
Pe conjugated anti f4 80, by BD (2 mentions)
Cd45 pe, by BD (1 mentions)
Cd11b pe, by BD (1 mentions)
Cd44 pe, by BD (1 mentions)
Cd106 fitc, by BD (1 mentions)
Fitc conjugated rat igg2a, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Pe conjugated anti cd11c, by BD (1 mentions)
4 protocols using pe conjugated rat igg2b
1
Immune Cell Dynamics in Malaria Infection
2
Flow Cytometry Analysis of ADSCs
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ADSCs were trypsinized, washed and suspended in 100 μl of ice-cold PBS containing 0.5% BSA. Rat anti-mouse antibodies (FITC-CD106, FITC-Ly-6A/E (Sca-1), PE-CD45, PE-CD44, and PE-CD11b; BD Pharmingen) were added for 30 min incubation at RT. PE-conjugated Rat IgG2b and FITC-conjugated Rat IgG2a (BD Pharmingen) were used as controls at concentrations recommended by the manufacturer. The cells were analyzed by flow cytometry using FACSCalibur (BD Biosciences) with the BD Cell-Quest_Pro version 4.0.1 software (BD Biosciences).
3
Phenotyping Peritoneal Immune Cells
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6-wk-old BALB/cJRj mice (Charles River) were i.p. infected with 105 parasites and sacrificed on days 4 and 7 p.i. Immediately after being killed, mice were peritoneally lavaged with PBS, and recovered lavage fluid was centrifuged at 500 g for 8 min. Peritoneal cells were washed in stain buffer (PBS containing 2% FBS and 0.1 mM EDTA), and cells were then pretreated at 4°C for 1 h with mAb 2.4G2 to block nonspecific binding to Fcγ receptors. Thereafter, cells were incubated for 1 h with fluorescently conjugated antibodies for cell surface markers from BD: PE-conjugated anti-CD11b, PE-conjugated anti-Gr1, PE-conjugated anti-CD11c, and APC-conjugated anti–Ly-6G. Isotype controls consisted of PE-conjugated rat IgG2b, PE-conjugated hamster IgG1, and APC-conjugated rat IgG2a, provided by BD. Analysis of stained cells was performed with a FACSCalibur flow cytometer (BD). For all samples, 100,000 cells were analyzed for plot generation.
4
Immune Cell Dynamics in Malaria Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
6-week-old BALB/cJRj mice (Charles River) were i.p. infected with 5 x 105 parasites and sacrificed on days 2, 4, and 7 p.i. Immediately after being killed, mice were peritoneally lavaged with PBS, and recovered lavage fluid was centrifuged at 500 g for 8 min. Peritoneal cells were washed once with PBS. Harvested cells were suspended in stain buffer (PBS containing 2% FBS and 0.1 mM EDTA), seeded in 96-well plates (106 cells/100 μl), and pretreated with FcBlock (clone 2.4G2; BD Biosciences) for 30 min at 4°C. Cells were then incubated with fluorescently conjugated antibodies for cell surface markers from BD Biosciences: PE-conjugated anti-CD11b, PE-conjugated anti-Gr1 (clone RB6-8C5), PE-conjugated anti-F4/80, and APC-conjugated anti-Ly6G. Isotype controls consisted of PE-conjugated rat IgG2b, PE-conjugated rat IgG2a, and APC-conjugated rat IgG2a, provided by BD Biosciences. Analysis of stained cells was performed with a FACSCalibur flow cytometer (BD Biosciences). For all samples, 100,000 cells were analyzed for plot generation.
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