Gensys 6 uv vis spectrophotometer

Measurements of cell density were performed by measuring absorbance at 600 nm using a Gensys 6 UV-Vis spectrophotometer (Thermo Fisher). Measurements of cell density and cell size were performed using a Coulter Z2 Particle Count and Size Analyzer (Beckman Coulter) with a 100- $\mathrm{\mu }$ m aperature. For comparative growth assays, cells were spotted onto relevant solid growth media. Cell spotting was performed by dilution of a stationary phase culture to an initial OD₆₀₀ of 1.0, followed by 10-fold serial dilutions. All dilutions were then spotted onto solid media using a Replica Plater for 96-well Plate, either the 8 × 6 or 12 × 8 array as needed (Sigma-Aldrich). Plates were incubated at indicated temperatures for indicated times as noted in the figures and legends. At least 3 independent biological replicates were performed on different days for spotting assays shown in figures, and a representative image is shown.

Measurements of cell density were performed by measuring absorbance at 600 nm using a Gensys 6 UV-Vis spectrophotometer (Thermo Fisher). Measurements of cell density and cell size were performed using a Coulter Z2 Particle Count and Size Analyzer (Beckman Coulter) with a 100- $\mathrm{\mu }$ m aperature. For comparative growth assays, cells were spotted onto relevant solid growth media. Cell spotting was performed by dilution of a stationary phase culture to an initial OD₆₀₀ of 1.0, followed by 10-fold serial dilutions. All dilutions were then spotted onto solid media using a Replica Plater for 96-well Plate, either the 8 × 6 or 12 × 8 array as needed (Sigma-Aldrich). Plates were incubated at indicated temperatures for indicated times as noted in the figures and legends. At least 3 independent biological replicates were performed on different days for spotting assays shown in figures, and a representative image is shown.