NIH3T3 and MCF7 cells were kindly provided by Stem Cell Bank, Chinese Academy of Sciences (China), and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% foetal bovine serum (FBS, Gibco) and antibiotic (1% penicillin/streptomycin (P/S)). HEK293F cell line was a generous gift from Qilin Zhang’s laboratory of Tsinghua University and was cultured in SMM 293-TI (Sino Biological, China) with 0.5% FBS and 1% P/S. All cells were maintained in a 5% CO2 humidified atmosphere at 37°C. Pertuzumab and trastuzumab monoclonal antibodies were kindly provided by Zhejiang Hisun Pharmaceutical Co., Ltd. (China).
Smm 293 ti
Manufactured by Sino Biological
Sourced in China
The SMM 293-TI is a cell line developed by Sino Biological for use in recombinant protein expression. It is derived from human embryonic kidney (HEK) 293 cells and is engineered to express the SV40 large T antigen, which enables it to be used for transient transfection experiments.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Freestyle 293 f, by Thermo Fisher Scientific (2 mentions)
Dmem basic, by Thermo Fisher Scientific (2 mentions)
Sf 900 3 sfm medium, by Thermo Fisher Scientific (2 mentions)
Ad293 cells, by Agilent Technologies (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Sino Biological (1 mentions)
Sf9 insect cells, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Sino Biological (1 mentions)
Penicillin, by Sino Biological (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Hek293ft cells, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Solarbio (1 mentions)
Streptomycin, by Solarbio (1 mentions)
Sim sf, by Sino Biological (1 mentions)
Sim hf, by Sino Biological (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Penicillin streptavidin, by Thermo Fisher Scientific (1 mentions)
5 protocols using smm 293 ti
1
Culturing NIH3T3, MCF7, and HEK293F cells
2
Cell Culture Conditions for FreeStyle 293 F, Sf9, and AD293
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FreeStyle 293 F (Thermo Fisher Scientific) suspension cells were cultured in SMM 293-TI (Sino Biological Inc.) supplemented with 1% FBS at 37 °C, with 6% CO2 and 70% humidity. Sf9 insect cells (Thermo Fisher Scientific) were cultured in Sf-900 III SFM medium (Thermo Fisher Scientific) at 27 °C. AD293 cells (Agilent) were cultured in DMEM basic (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) at 37 °C, with 6% CO2 and 70% humidity.
3
Establishing and Purifying HEK293FT Cell Line
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HEK293FT cells (Invitrogen, R70007) were cultured in DMEM medium (Hyclone, SH30243.01B) supplemented with 10% fetal bovine serum (FBS) (Biological Industries, 04–001–1 A), 1x glutaMAX (Gibco, 35050-061), 100 U/ml penicillin and 10 mg/ml streptomycin (Solarbio, P1400), at 37 °C in a 5.5% CO2 humidified atmosphere for stable cell line establishment. When establishment completed, cells were transferred to flanking bottles in SMM 293-TI (Sino Biological inc, M293TI-1), containing 1% FBS, 1x glutaMAX, 100 U/ml penicillin and 10 mg/ml streptomycin for purification. Cell lines were not authenticated or tested for mycoplasma contamination.
4
Cell Culture Protocols for Diverse Cell Lines
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FreeStyle 293 F (Thermo Fisher Scientific) suspension cells were cultured in SMM 293-TI (Sino Biological Inc.) supplemented with 1% FBS at 37 °C, with 6% CO2 and 70% humidity. Sf9 insect (Thermo Fisher Scientific) cells were cultured in Sf-900 III SFM medium (Thermo Fisher Scientific) at 27 °C. AD293 cells (Agilent) were cultured in DMEM basic (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) at 37 °C, with 6% CO2 and 70% humidity.
5
Cell Culture Conditions for Diverse Cell Lines
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Sf21 and High Five cells were maintained using a non-humidified shaker at 27°C in the SIM-SF and SIM-HF media (Sino Biological), respectively. HEK293F cells were cultured in a humidified shaker with 5% CO 2 and 55% humidity at 37°C in SMM 293T-I (Sino Biological).
HeLa and HEK293T cells were cultured in DMEM (Gibco) supplemented with 1% penicillinstreptomycin (Gibco) and 10% fetal bovine serum (FBS, Gibco) in a humidified incubator at 37 °C with 5% CO 2 . Human Jurkat T cells were cultured in RPMI-1640 medium supplemented with 2 mM GlutaMAX (Gibco), 15% FBS, 10 mM HEPES (Gibco), 1 mM Sodium Pyruvate (Gibco), 1% MEM Non-Essential Amino Acids Solution (100×, Gibco), and 1% penicillinstreptavidin at 37 °C and 5% CO 2 .
HeLa and HEK293T cells were cultured in DMEM (Gibco) supplemented with 1% penicillinstreptomycin (Gibco) and 10% fetal bovine serum (FBS, Gibco) in a humidified incubator at 37 °C with 5% CO 2 . Human Jurkat T cells were cultured in RPMI-1640 medium supplemented with 2 mM GlutaMAX (Gibco), 15% FBS, 10 mM HEPES (Gibco), 1 mM Sodium Pyruvate (Gibco), 1% MEM Non-Essential Amino Acids Solution (100×, Gibco), and 1% penicillinstreptavidin at 37 °C and 5% CO 2 .
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