Mc170 hd imaging system camera

hWJ-MSCs seeded at density of 3000 cells/cm² in 24-well plates were exposed for 24 h to CB (0.1, 1 and 3 µM) or DMSO. Every experimental condition was assayed in technical duplicate. At the end of the treatment, a morphological analysis was performed, and representative images of cells were acquired under a light microscope (Leica Labovert FS Inverted Microscope; Wetzlar, Germany) with a Leica MC170 HD Imaging System Camera (Wetzlar, Germany).

For cell migration analysis, hWJ-MSCs were seeded into 24-well plates. After cells reached 100% confluence, a scratch, which consists of two straight lines that crossed in the middle of the well, was made by a pipette tip. Then, hWJ-MSCs were cultured for 24 h in absence or presence of CB (0.1, 1, and 3 µM). Images were acquired at 0 and 24 h from the scratch using a light microscope (Leica Labovert FS Inverted Microscope; Wetzlar, Germany) equipped with a Leica MC170 HD Imaging System Camera (Wetzlar, Germany). To evaluate the reversible effect of CB, after 24 h of treatment, the medium was replaced with standard fresh medium in all samples, and then cells were cultured for additional 24 h before images acquisition.