Raw264

The CT26, MC38, 4T-1, and RAW264.7 cell lines were purchased from the National Infrastructure of Cell Line Resource (Beijing, China) and kept by our laboratory. The cells were cultured in a constant temperature incubator containing 5% CO₂ at 37°C.
OH2 was provided by Binhui Biopharmaceutical Co., Ltd. (Wuhan, China). The virus was an attenuated OH2 derived from the wild-type HSV-2 strain HG52 deleted the ICP47 gene and ICP34.5 gene [18 (link), 19 (link)].

The mouse melanoma cell line B16-F10-Luc-G5 (B16 cells) and the mouse Lewis lung cancer cell line LL/2-Luc-M38 (LL/2 cells) were purchased from Shanghai Genomics Technology, Ltd. FaDu human pharyngeal squamous carcinoma cells were kindly donated by Prof. Israel Vlodavsky (Technion Integrated Cancer Center, Rappaport Faculty of Medicine). The human ovarian cancer cell line A2780 was purchased from Hunan Fenghui Biological Ltd. The mouse mononuclear macrophage leukemia cell line RAW 264.7 was obtained from the National Infrastructure of Cell Line Resource (NICR, Beijing, China). B16, A2780 and RAW 264.7 cells were incubated in RPMI1640 (Gibco, Invitrogen, Grand Island, USA) with 10% FBS (Gibco, Invitrogen), 100 U/mL penicillin, and 100 mg/mL streptomycin at 37 °C in 5% CO₂, while LL/2 and FaDu cells were cultured in DMEM under the same conditions.
C57BL/6N mice were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd. Six- to eight-week-old male mice were used for the in vivo assay, while those aged 18–20 weeks were used for washed platelet preparation. This study was approved by the Animal Ethics Committee of the Laboratory Animal Center, Beijing Hospital of Traditional Chinese Medicine (No.2019120202).

The standard bacterial strains used in this study included N. farcinica IFM10152, N. asteroids DSM43757T, N. cyriaciegeorgoca DSM44484T, N. brasiliensis DSM43758T, N. otitidiscaviarum DSM43242T, N. transvalensis DSM43405T, N. veterana DSM44445T, and N. nova DSM44481T. All the Nocardia strains were procured from the German Resource Centre for Biological Materials and cultured in BHI medium (Oxoid Ltd., Hants, UK) at a 37 °C incubator. Escherichia coli BL21(DE3) cells were procured from TransGen Biotech and cultured in an LB medium containing 50 µg/mL kanamycin. The pET30a plasmid was constructed in our laboratory and used to express N. farcinica NFA49590 in E. coli. The mouse cell line RAW264.7 (National Infrastructure of Cell Line Resource, Beijing, China) was cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS).