Masshunter workstation b 07

A Shimadzu Nexis 2030 gas chromatograph equipped with a programmed split/splitless injector and an AOC 6000 multifunction autosampler (Shimadzu, Kyoto, Japan), and a Shimadzu 8040 138 NX tandem mass spectrometry (Shimadzu, Kyoto, Japan) were used to perform gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) confirmation. An SH-Rxi-5Sil MS (30 m 140 × 0.25 mm id × 0.25 μm film) capillary column was used. A 1290 Infinity UHPLC system was linked to a 6495 Triple Quadrupole LC-MS/MS device added with a jet stream EI source (Agilent, Santa Clara, CA, USA). Data were acquired and analyzed on an Agilent MassHunter Workstation B.07.00. Chromatographic isolation was finished on an Agilent ZORBAX Eclipse Plus C₁₈ column (50 mm × 2.1 mm, 1.8 μm) with gradient elution.
Samples were prepared using a GENIUS 3 vortex agitator (IKA, Stauffen, Germany), a CL31R multispeed refrigerated centrifuge (Thermo Scientific, Waltham, MA, USA), a WD12 water bath nitrogen blowing instrument (Aosheng Instrument, Hangzhou, China), and a CK2000 high-throughput tissue grinder (Thmorgan Biotechnology, Beijing, China).

Phenolic compounds were identified from the ethyl acetate fraction of leaves of Tradescantia pallida by following the method of Emir et al. [27 (link)], with slight modifications. The metabolites were separated using HPLC (Agilent 1260 Infinity II, Santa Clara, CA, USA) equipped with triple quadrupole LC/MS/MS (6470, Agilent Technologies, Santa Clara, CA, USA). The metabolites were separated on an RP-18 column (100 mm × 2.1 mm, 1.8 μm) with an injection volume of 10 µL and a flow rate of 0.4 mL/min. The mobile phase consisted of water and formic acid with 0.1% v/v as solvent A and acetonitrile with 0.1% v/v as solvent B. The elution was carried out using gradient mode (Table 1). A mass spectrum was obtained by using negative ESI mode at 5000 capillary voltage in the range of 50–3000 m/z. The nebulization pressure used was 35 psi. The gas temperature was 300 °C with a gas flow of 9 L/min. The sheath gas heater was at 250 °C with sheath gas flow of 12 arb. The total run time was 66 min, and data were processed using MassHunter workstation B.07.00 (Agilent Technologies, Santa Clara, CA, USA). The tentative compounds were identified with reference standards.