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Ultrascan 4k 4k camera

Manufactured by Ametek

The Ultrascan 4k × 4k camera is a high-resolution imaging device designed for laboratory applications. It features a 4,096 × 4,096 pixel sensor, enabling the capture of detailed images. The camera's core function is to provide users with the ability to acquire high-quality, high-resolution data for their research or analysis needs.

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4 protocols using ultrascan 4k 4k camera

1

Automated TEM Imaging and 3D Reconstruction

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Sections were examined using an FEI Tecnai 20 TEM operated at 80kV. Images were acquired with a Gatan Ultrascan 4k × 4k camera. Serial section images were acquired as montages of 4k × 4k micrographs using the automated acquisition software Leginon. Montaging and 3D reconstruction were carried out using ImageJ-TrakEM254 , 55 (link).
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2

Automated TEM Imaging and 3D Reconstruction

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Sections were examined using an FEI Tecnai 20 TEM operated at 80kV. Images were acquired with a Gatan Ultrascan 4k × 4k camera. Serial section images were acquired as montages of 4k × 4k micrographs using the automated acquisition software Leginon. Montaging and 3D reconstruction were carried out using ImageJ-TrakEM254 , 55 (link).
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3

Transmission Electron Microscopy of Larval Samples

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Larva were fixed with 2% glutaraldehyde in 0.1 M Na cacodylate
buffer, pH 7.3, for 2 hours at room temp, rinsed in buffer, and post-fixed
with 1% OsO4 in a buffer for 1 hour at room temperature. The post-fixed
samples were then rinsed with water, stained en bloc with
5% uranyl acetate in water, dehydrated in an ethanol series followed by
propylene oxide, and embedded in Eponate 12 resin (Ted Pella, Inc., Redding,
CA). 50 nm sections were cut with a Leica UCT ultramicrotome using a Diatome
diamond knife, picked up on slot grids with Pioloform films, stained with
uranyl acetate and Sato’s lead, and examined with an FEI T12 TEM at
120 kV equipped with a Gatan Ultrascan 4k × 4k camera.
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4

Transmission Electron Microscopy Tissue Prep

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Animals were fixed with 2% glutaraldehyde in 0.1 M Nacacodylate buffer, pH 7.3, for 2 h at room temp, rinsed in buffer, and post-fixed with 1% OsO4 in a buffer for 1 h at room temp. The post-fixed samples were then rinsed with water, stained en bloc with 5% uranyl acetate in water, dehydrated in ethanol and propylene oxide, and embedded in Eponate 12 resin (Ted Pella, Inc., Redding, CA). 50 nm sections were cut with a Leica UCT ultramicrotome using a Diatome diamond knife, picked up on slot grids with Pioloform films, stained with uranyl acetate and Sato’s lead, and examined with an FEI T12 TEM at 120 kV equipped with a Gatan Ultrascan 4k × 4k camera.
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