Micromass quattro premier xe quadrupole tandem mass spectrometer

Shoot samples were lyophilized with a Labconco freeze drier system (Kansas, USA) at − 50 °C for three days, and ~ 50 mg of dried samples were used for hormone profiling. Phytohormone analysis was performed at the National Research Council of Canada, Saskatoon, SK on a fee-for-service basis. UPLC/ESI–MS/MS of Waters ACQUITY UPLC system, coupled to a Waters Micromass Quattro Premier XE quadrupole tandem mass spectrometer via a Z-spray interface was used. Quantification of phytohormones was performed using deuterium labeled internal standards^{32 (link)}.

For quantification of salicylates and jasmonates, 500 mg of fresh leaf tissue was employed. For quantification of ACC, 1 g of frozen leaf biomass powder was lyophilized for 48 h to obtain 50 mg of dried tissue. Once weighted, samples were placed on dry ice and shipped to Dr. Irina Zaharia at the National Research Council of Canada located in Saskatoon, Saskatchewan, Canada. Hormone quantification was performed using high‐performance liquid chromatography–electrospray ionization–tandem mass spectrometry (HPLC‐ESI‐MS/MS), using the ACQUITY UPLC system equipped with a binary solvent delivery manager and a sample manager (Waters). This system was also coupled to a Micromass Quattro Premier XE quadrupole tandem mass spectrometer (Waters). MassLynx and QuanLynx softwares (Micromass) were used for data acquisition and analysis. Quantification data was obtained from comparison with standard‐derived calibration curves.