Anti sqstm1

Manufactured by BD

Anti-SQSTM1 is a laboratory reagent used in research applications. It is a monoclonal antibody that binds to the SQSTM1 protein, also known as p62. SQSTM1 is involved in various cellular processes, including autophagy and signaling pathways. The Anti-SQSTM1 reagent can be used to detect and study the SQSTM1 protein in biological samples.

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Lab products found in correlation

Nb100 2220, by Novus Biologicals (1 mentions) Irdye 680lt goat anti mouse, by LI COR (1 mentions) Anti β tubulin, by Novus Biologicals (1 mentions) Lamp1, by Abcam (1 mentions) Orp150, by Abcam (1 mentions) Cthrc1, by Abcam (1 mentions) Eif2α, by Santa Cruz Biotechnology (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Spautin 1, by Merck Group (1 mentions) Anti myd88, by Cell Signaling Technology (1 mentions) Anti lc3, by Cell Signaling Technology (1 mentions) 3 methyladenin, by Merck Group (1 mentions) Filipin, by Merck Group (1 mentions) Monoclonal anti β actin, by Abcam (1 mentions) Anti lc3, by BD (1 mentions) Anti sqstm1, by Abnova (1 mentions) Sucrose, by Merck Group (1 mentions)

3 protocols using anti sqstm1

Multifaceted Western Blot Analysis

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A combination of rabbit polyclonal anti-MAP1LC3B (Novus, NB100-2220) at 1:5000 and mouse monoclonal anti-β-Tubulin (DSHB, E7) at 1:2000 dilution was used for Western blotting of chloroquine treated cell lysates.
A combination of rabbit polyclonal anti-HDAC2 (Thermo Scientific, PA1-861) at 1:3000 and mouse monoclonal anti-β-Tubulin (DSHB, E7) at 1:2000 was used as positive controls for the nuclear and cytoplasmic fractions, respectively. A combination of mouse monoclonal anti-GFP (Clontech, 632381) at 1:2000 and rabbit polyclonal anti-MAP1LC3B (Novus, NB100-2220) at 1:5000 was used to analyze the IP fractions. For validation of the MudPIT results, Western blots were probed with either mouse monoclonal anti-SQSTM1 (BD, 610832) at 1:2000 dilution or goat polyclonal anti-MAP1B (Santa Cruz, sc-8970) at 1:100 dilution in combination with rabbit polyclonal anti-MAP1LC3B (Novus, NB100-2220) at 1:5000.
Secondary antibodies used for Western blotting included goat anti-rabbit IRDye-800CW (LI-COR, 926-32211), goat anti mouse IRDye 680 LT (LI-COR, 926-68020), donkey anti-goat IRDye-800CW (926-32214), donkey anti-rabbit IRDye 680 LT (926-68023).

Kraft L.J., Manral P., Dowler J, & Kenworthy A.K. (2016). Nuclear LC3 associates with slowly diffusing complexes that survey the nucleolus. Traffic (Copenhagen, Denmark), 17(4), 369-399.

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Western Blot and Immunofluorescence Antibodies

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The following antibodies were used for WB and/or IF as indicated: SIL1 (Proteintech #24110-1-AP); ATF4 (Cell Signaling #11815); ATF6B (Proteintech #15794-1-AP); EIF2α (Santa Cruz #sc-11386); pho-EIF2α (Cell Signaling #3597s); PDI (Assay Design #SPA-891); BiP (BD #610979); ORP150 (Abcam #EP5891); LC3B (Cell Signaling #2775); NCL (Cell Signaling #87792S); NPM (Cell Signaling #3542S); CTHRC1 (Abcam #ab85739); Anti SQSTM1 (BD Bioscience #610833); LAMP1 (Abcam #ab25630); CNX (Cell Signaling #2679S); NES (Millipore #AB5922); CAV-1 (Cell Signaling #3238); and GAPDH (Santa Cruz #sc-32233). Hoechst #33342 from Life Technology was used to counterstain nuclei. For PCR amplification of the spliced and unspliced form of human XBP1, the following primers were used: FW 5′-AGCTCAGACTGCCAGAGATCG-3′; REV 5′-AATCCATGGGGAGATGTTCTA-3′ [35 (link)]. Powders, buffers, and other commonly used reagents were supplied by Sigma unless otherwise noted and were of analytical grade or higher.

Potenza F., Cufaro M.C., Di Biase L., Panella V., Di Campli A., Ruggieri A.G., Dufrusine B., Restelli E., Pietrangelo L., Protasi F., Pieragostino D., De Laurenzi V., Federici L., Chiesa R, & Sallese M. (2021). Proteomic Analysis of Marinesco–Sjogren Syndrome Fibroblasts Indicates Pro-Survival Metabolic Adaptation to SIL1 Loss. International Journal of Molecular Sciences, 22(22), 12449.

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Immunoblot and Immunofluorescence Analysis of Autophagy

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For immunoblot analysis, anti-SQSTM1, anti-LC3 and anti-BECN1 were purchased from BD Biosciences and Sigma Aldrich, respectively. For immunofluorescence studies, anti-LC3 was purchased from Cell Signaling and anti-SQSTM1 from Abnova. Anti-TLR2 was from Genetex and anti-MyD88 was from Cell Signaling. Monoclonal anti- β-actin was provided by Abcam and a polyclonal anti- GAPDH was from Ambion Life Technologies. Antibodies directed against gD, ICP0, phospho-p38α and histone H3 were from Santa Cruz Biotechnology; the antibody against ICP8 was kindly provided by Pr. Bernard Roizman. Horseradish peroxidase anti-rabbit and anti-mouse antibodies were from Santa Cruz Biotechnology. Tetramethyl rhodamine isothiocyanate (TRITC)-conjugated goat anti-rabbit antibody was from Jackson and Alexa Fluor 350 donkey anti-mouse antibody was from Invitrogen. Spautin-1 (10 μM), 3-methyladenin (10 mM), filipin (2.5 μg/ml) and sucrose (0.45 M) were from Sigma Aldrich.

Siracusano G., Venuti A., Lombardo D., Mastino A., Esclatine A, & Sciortino M.T. (2016). Early activation of MyD88-mediated autophagy sustains HSV-1 replication in human monocytic THP-1 cells. Scientific Reports, 6, 31302.

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