Il 17

Sodium butyrate (Figure 1(a); HPLC ≥ 98.5% purity) was obtained from Sigma-Aldrich (Shanghai) Trading Co. Ltd. (China). TNF-α, IL-1β, IL-6, IL-10, TGF-β, and IL-17 assay kits were purchased from Shanghai Enzyme-linked Biotechnology Co. Ltd. (Shanghai, China). Primary antibodies, including anti-iNOS (Cell Signaling, 13120S), anti-Arg-1 (Cell Signaling, 93668S), anti-ROR gamma(t) (Invitrogen, 14-6988-82), anti-FoxP3 (Beijing Biosynthesis Biotechnology Co. Ltd., Beijing, China, bs-10211R), anti-β-actin (Proteintech, 20536-1-AP), and anti-insulin (Abcam, ab181547). Goat anti-rabbit secondary antibodies (cat. no. 10285-1-AP) were obtained from ProteinTech Group, Inc. (Chicago, IL, USA).

Reagent of IL-6 (batch number: E20191204-30219a), TNF-α (batch number: e20191201-31063a), IL-17 (batch number: e20191202-30201a), TGF-β (batch number: e20191206-31072a), and IL-10 (batch number: e20191203-30194a) were purchased from Shanghai Enzyme-Linked Biotechnology Co., Ltd. Blood was collected from the rat heart, centrifuged at 4°C at 3000 r/min for 15 min, and serum was collected and stored at -80°C. The cortical tissue and colon tissue of the rat separately were removed from the ice and diluted after homogenization. The contents of proinflammatory factors IL-6, TNF-α, and IL-17and anti-inflammatory factors TGF- and IL-10 in the cerebral cortex and serum of rats were detected by ELISA.