Lsm 5.0 image browser software

Images of tissues were acquired either by a charge-coupled device camera system (DP73; cellSens Standard 1.6; Olympus) attached to a microscope (BX50; Olympus) or through a confocal microscope system (LSM510; LSM 5.0 Image Browser software; Carl Zeiss, Germany). Images were analysed by Photoshop CS5 Extended (Adobe Systems, San Jose, CA) and with software for the image acquisition systems. Figures were prepared using Photoshop CS5 Extended. Image, brightness, contrast and sharpness were adjusted according to the journal's guidelines.

Transgenic newts were monitored under a fluorescence dissecting microscope (M165 FC) which was installed with filter sets specific to AmCyan (CFP; Exciter: 436/20 nm; Emitter: 480/40 nm; Leica Microsystems), YFP (YFP; Exciter: 500/20 nm; Emitter: 535/30 nm; Leica Microsystems) and mCherry (Exciter: XF1044, 575DF25; Emitter: XF3402, 645OM75; Opto Science, Tokyo, Japan). Images were taken by a digital camera system (EOS Kiss x7i; Canon, Tokyo, Japan) attached to the microscope. Eyeball sections were examined on a fluorescence microscope (BX50; Olympus, Tokyo, Japan) which was also installed with the same filter sets as well as standard filter sets for EGFP, Texas red and DAPI. Their images were taken by a charge-coupled device camera system (DP73; cellSens Standard 1.6; Olympus) attached to this microscope (BX50; Olympus) or by a confocal microscope system (LSM510; LSM 5.0 Image Browser software; Carl Zeiss, Germany). Images were analyzed with software for the image acquisition systems and in Photoshop CS5 Extended (Adobe Systems, San Jose, CA). Figures and panels were prepared using Photoshop CS5 Extended. Images, brightness, contrast and sharpness were adjusted according to the journal’s guidelines.