3 4 5 dimethylthiazolyl 2 2 5 diphenyltetrazolium bromide mtt griess reagent

Agarose (Standart Low-MR) was obtained from BioRad Laboratories (Richmond, CA, USA). 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT), Griess reagent, N-ω-Nitro-l-arginine methyl ester hydrochloride (L-NAME), and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were purchased from Sigma Chemical Company, (St. Louis, MO, USA). Dulbecco′s modified Eagle′s medium (DMEM) and fetal bovine serum (FBS) were obtained from CULTILAB (Campinas, SP, Brazil). Penicillin and streptomycin were obtained from Gibco (Fort Worth, TX, USA). SYBR Green Master Mix and High-Capacity cDNA Reverse Transcription Kit were from Applied Biosystems (Foster City, CA, USA). The ReliaPrep RNA Cell Miniprep System was purchased from Promega (Madison, WI, USA). DNAse were from Ambion (Life technologies, Carlsbad, CA, USA). Cytokine analysis kits were purchased from BD Biosciences (San Jose, CA, USA). Lipopolysaccharide (LPS, Escherichia coli 055: B5) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). All other solvents and chemical products were of analytical grade.

Potassium ferricianyde, ferrous sulfate II, trichloroacetic acid, and sulfuric acid were purchased from Merck (Darmstadt, Germany). Nitro Blue Tetrazolium (NBT), monosaccharides, diaminoethanetetraacetic acid (EDTA), ascorbic acid, methionine, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT), Griess reagent, pyrocatechol violet, riboflavin, and ammonium molybdate were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Sodium bicarbonate, non-essential amino acids, and phosphate buffered saline (PBS) were purchased from Invitrogen Corporation (Burlington, ON, Canada). Dulbecco′s modified Eagle′s medium (DMEM) and fetal bovine serum (FBS) were obtained from CULTILAB (Campinas, SP, Brazil). Penicillin and streptomycin were obtained from Gibco (Fort Worth, TX, USA). All other solvents and chemicals were of analytical grade.
The HEK-293 cells (ATCC^® CRL-1573™) were grown in a DMEM medium with 10% FBS, 100 μg/mL streptomycin and 100 IU/mL Penicillin. The cells were cultured in DMEM supplemented with FBS (10% v/v) and antibiotics (100 U/mL Penicillin and 100 μg/mL streptomycin), at 37 °C in a humidified atmosphere with 5% CO₂. For maintenance of the cells, the culture medium was changed every three days, and the cells were further subcultured using trypsin/EDTA purchased from CULTILAB (Campinas, SP, Brazil).