Proteins of cell lysates or tissue homogenates were separated by SDS–PAGE according to their molecular weight using Tris/glycine as electrophoresis buffer and were transferred onto polyvinylidene fluoride membranes (Carl Roth GmbH, Karlsruhe, Germany) using CAPS buffer. After blocking, membranes were hybridized with respective primary antibodies. Membranes were washed, incubated with respective secondary horseradish-peroxidase (HRP)-conjugated antibody and detected using ECL2 Western blotting substrate (Thermo Scientific, Waltham, MA). Antibodies used were rabbit anti-HSL, rabbit anti-phospho-HSL (S660), and rabbit anti-GAPDH (all from Cell Signaling, Danvers, MA, USA), rabbit anti-CGI-58 (Abnova, Heidelberg), mouse anti-β-Actin (Santa Cruz, Santa Cruz, CA, USA), rabbit anti-alpha smooth muscle actin (α-SMA, Pierce, Thermo Scientific), HRP-linked sheep-anti mouse antibody, (GE Healthcare Amersham, Buckinghamshire, UK), and HRP-linked rat-anti rabbit antibody (Dako, Glostrup, Denmark).
Hrp linked sheep anti mouse antibody
Manufactured by GE Healthcare
Sourced in United Kingdom
The HRP-linked sheep-anti mouse antibody is a laboratory reagent used in various immunoassay techniques. It consists of an antibody raised in sheep that specifically binds to mouse immunoglobulins, and is conjugated with the enzyme horseradish peroxidase (HRP). This conjugation allows for the detection and quantification of target mouse proteins or antigens in a sample when used in appropriate assay protocols.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene fluoride membrane, by Carl Roth (2 mentions)
Rabbit anti phospho hsl s660, by Cell Signaling Technology (2 mentions)
Rabbit anti cgi 58, by Abnova (2 mentions)
Hrp linked rat anti rabbit antibody, by Agilent Technologies (2 mentions)
Ecl2 western blotting substrate, by Thermo Fisher Scientific (2 mentions)
Rabbit anti hsl, by Cell Signaling Technology (2 mentions)
Mouse anti β actin, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (2 mentions)
2 protocols using hrp linked sheep anti mouse antibody
1
Western Blot Analysis of Lipid Metabolism Proteins
2
Western Blot Analysis of Lipid Metabolism Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Proteins of cell lysates or tissue homogenates were separated by SDS–PAGE according to their molecular weight using Tris/glycine as electrophoresis buffer and were transferred onto polyvinylidene fluoride membranes (Carl Roth GmbH, Karlsruhe, Germany) using CAPS buffer. After blocking, membranes were hybridized with respective primary antibodies. Membranes were washed, incubated with respective secondary horseradish-peroxidase (HRP)-conjugated antibody and detected using ECL2 Western blotting substrate (Thermo Scientific, Waltham, MA). Antibodies used were rabbit anti-HSL, rabbit anti-phospho-HSL (S660), and rabbit anti-GAPDH (all from Cell Signaling, Danvers, MA, USA), rabbit anti-CGI-58 (Abnova, Heidelberg), mouse anti-β-Actin (Santa Cruz, Santa Cruz, CA, USA), rabbit anti-alpha smooth muscle actin (α-SMA, Pierce, Thermo Scientific), HRP-linked sheep-anti mouse antibody (GE Healthcare Amersham, Buckinghamshire, UK), and HRP-linked rat-anti rabbit antibody (Dako, Glostrup, Denmark).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!