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Anti v5 tag antibody

Manufactured by Cell Signaling Technology
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Sourced in United States

The Anti–V5-tag antibody is a laboratory reagent used to detect and purify proteins tagged with the V5 epitope. The antibody binds specifically to the V5 tag, allowing for identification and isolation of the tagged protein in various experimental procedures.

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Lab products found in correlation

Anti phospho egfr y1068 antibody, by Cell Signaling Technology (1 mentions) Anti p44 42 mapk antibody, by Cell Signaling Technology (1 mentions) Anti braf antibody, by Santa Cruz Biotechnology (1 mentions) Protein g agarose bead, by Thermo Fisher Scientific (1 mentions) P85 antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

V5 tag antibody Anti-V5-tag V5-tag Anti-V5 antibody V5 antibody Anti-V5

2 protocols using anti v5 tag antibody

1

Comprehensive Antibody Detection Protocol

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Anti–V5-tag antibody (#13202), anti–phospho-p44/42 MAPK (Thr202/Tyr204) antibody (#9101L), anti- p44/42 MAPK antibody (#9102), anti–phospho-MEK1/2 (Ser217/221) antibody (41G9) (#9154), anti-MEK1 (61B12) antibody (#2352), anti–phospho-Akt (Thr308) antibody (#9275), anti–phospho-Akt (Ser473) antibody (#4060), anti-Akt antibody (pan) (C67E7, #4691), anti–phospho-EGFR (Y1068) antibody (#3777), anti-EGFR receptor (D38B1) antibody (#4267), anti–phospho-HER3/ErbB3 (Tyr1289) (21D3) antibody (#4791), anti-HER3/ERBB3 (D22C5) XP antibody (#12708), anti-FoxC1 (D8A6) antibody (#8758), and anti-KLF5 (D7S3F) antibody (#51586) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-ARAF antibody (catalog no. sc-408), anti-BRAF antibody (catalog no. sc-5284), and anti-CRAF antibody (catalog no. sc-133) were obtained from Santa Cruz. Anti-vinculin antibody (catalog no. SAB4200080) was purchased from Sigma-Aldrich. Anti–M2-PK antibody (S-1) was purchased from Schebo Biotech AG. HRP-conjugated secondary antibodies for rabbit immunoglobulin G were obtained from Novex (Boston, MA, USA; A16096) and Thermo Fisher Scientific (#32460).
Mooz J., Riegel K., PS H., Sadanandam A., Marini F., Klein M., Werner U., Roth W., Wilken-Schmitz A., Tegeder I, & Rajalingam K. (2022). ARAF suppresses ERBB3 expression and metastasis in a subset of lung cancers. Science Advances, 8(11), eabk1538.
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2

V5-Tag Immunoprecipitation and Western Blot Analysis

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Cell lysates were harvested using ice cold lysis buffer (1% Triton X-100, 10% Glycerol, 100 mM NaCL, 50 mM HEPES, 100 mM Na3VO4, 10 mM NaF, 1 Roche Minitab) and rotated at 4°C for 1 h. Lysates were then clarified by spinning at 10,000 xg at 4°C for 15 min. Protein concentrations were measured using BCA standard curves (Pierce). One μg anti V5-Tag antibody (Cell signaling 13202) was added to 1 mg of clarified cell lysate and rotated at 4°C overnight. 50 μL of Protein G agarose beads (Life technologies 10003D) were added to the lysates and rotated at 4°C for 4 h. Tubes were placed on magnets, washed three times with lysis buffer, and eluted in 20 μL of LDS sample buffer. Lysates were subjected to immunoblot analysis with a p85 antibody (Cell signaling 4257). Unbound lysate was analyzed via western blot for loading controls. Immunoblots were analyzed using ImageJ software.
Croessmann S., Sheehan J.H., Lee K.M., Sliwoski G., He J., Nagy R., Riddle D., Mayer I., Balko J.M., Lanman R., Miller V.A., Cantley L.C., Meiler J, & Arteaga C.L. (2017). PIK3CA C2 domain deletions hyperactivate phosphoinositide 3-kinase (PI3K), generate oncogene dependence and are exquisitely sensitive to PI3Kα inhibitors. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(6), 1426-1435.
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