Total RNAs from control vein or from AVF were isolated using the RNeasy kit (Qiagen, Valencia, CA). Real-time RT-PCR was performed using Opticon real-time RT-PCR machine (MJ Research, Waltham, MA). The specificity of real-time RT-PCR was confirmed using agarose gel electrophoresis and melting-curve analysis.
Opticon real time rt pcr machine
Manufactured by Bio-Rad
The Opticon real-time RT-PCR machine is a laboratory instrument designed for quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) analysis. It is capable of detecting and quantifying nucleic acid sequences in real-time during the amplification process.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using opticon real time rt pcr machine
1
RNA Isolation and Real-time RT-PCR
2
RNA Isolation and Real-time RT-PCR
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