Bond refine kit

Manufactured by Leica

Sourced in Germany

The Bond Refine Kit is a laboratory-grade product designed for sample preparation and optimization. It provides essential tools and reagents to improve the quality and consistency of samples before further analysis. The kit includes materials to perform key steps in the sample preparation process, but does not make claims about specific applications or intended uses.

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Lab products found in correlation

Bond max system, by Leica (2 mentions) Bond epitope retrieval buffer, by Leica (1 mentions)

Close spelling variants of this product

Bond Polymer Refine Detection kit (459 citations) Bond Polymer Refine Red Detection Kit (48 citations) Bond Polymer Refine Kit (27 citations) Bond Refine Detection Kit (19 citations) BOND Polymer Refine DAB Detection kit (10 citations) Bond Refine polymer staining kit (10 citations) Bond Polymer Refine Detection Kit DC9800 (5 citations) Bond Polymer Refine Detection Kit DS9800 (3 citations) Bond Polymer Refine Red Kit (3 citations) Leica Bond Polymer Refine HRP kit (3 citations)

3 protocols using bond refine kit

Automated Quantification of Tumor Angiogenesis

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Paraffin sections were processed using Bond Epitope Retrieval Buffer, Bond Leica Automated Staining and Bond Refine Kit (Leica Microsystems). Antibody used for mouse tissues: anti-CD34 (MEC14.7; Santa Cruz Biotechnology), at a dilution of 1:100. All slides were counterstained with haematoxylin. Species- and subtype-matched antibodies were used as negative controls.
Quantification of blood vessels size was done using ImageJ software. Values were calculated based on the mean size of 5 randomly chosen CD34-stained blood vessels for each tumor in each genotype and based on pixel numbers.

Dulloo I., Hooi P.B, & Sabapathy K. (2015). Hypoxia-induced DNp73 stabilization regulates Vegf-A expression and tumor angiogenesis similar to TAp73. Cell Cycle, 14(22), 3533-3539.

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Immunostaining for Thyroglobulin in Thyroid Cancer

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After surgery, the thyroid and cervical lymph nodes were fixed in 10% neutral buffered formalin, and the specimens were embedded in paraffin. Serial sections (3-µm thick) were cut from each paraffin block.
For six patients with structural recurrence but undetectable Tg values determined by Tg-ECLIA and Tg-LC-MS/MS, immunostaining for human Tg (mouse monoclonal, 1D4, 1:200, Leica Biosystems Newcastle, United Kingdom) was performed using the Leica Bondmax system (Leica Microsystems, Wetzlar, Germany) and a Bond Refine Kit (Leica Microsystems), according to the manufacturer’s instructions.

Nishihara E., Hobo Y., Miyauchi A., Ito Y., Higuchi M., Hirokawa M., Ito M., Fukata S., Nishikawa M, & Akamizu T. (2021). Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinoma. European Thyroid Journal, 11(1), e210041.

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Immunohistochemical Analysis of Carcinoma Samples

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Immunohistochemistry was performed using 3.0-μm-thick, formalin-fixed, paraffin-embedded tissue specimens. The primary antibodies used for immunostaining and antigen retrieval methods are listed in Table 1. Staining was performed using the Leica Bondmax system (Leica Microsystems, Wetzlar, Germany) and Bond refine kit (Leica Microsystems, Wetzlar, Germany) according to the manufacturer's recommendations. Cases where >10.0% of the carcinoma cells exhibited moderate-to-strong staining were considered to be immunoreactive [9] .

Suzuki A., Hirokawa M., Takada N., Higuchi M., Tanaka A., Hayashi T., Kuma S, & Miyauchi A. (2018). Utility of monoclonal PAX8 antibody for distinguishing intrathyroid thymic carcinoma from follicular cell-derived thyroid carcinoma. Endocrine journal, 65(12).

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