10a system controller

All HPLC fingerprint analyses of the water extracts of BHJRST and the single remedies of BHJRST were performed on a HPLC instrument equipped with a Shimadzu 10A system controller (both from Shimadzu Corporation, Kyoto, Japan). The injection volume was 10 μL, the column was a Nacalai Cosmosil 5C₁₈-AR-II Waters (5 μm, 4.6 × 250 mm; Nacalai Tesque), the linear gradient was 10.0–90.0% B (A = MeOH, B = H₂O) in 60 min, detection was at 280 nm, and the flow rate was 0.8 mL/min.

A Shimadzu HPLC system, composed of an LC 10AD solvent pump, an SLC 10A system controller, and a CTO 10AS column oven, was employed. A Quattro LC triple quadrupole tandem MS/MS system (Micromass, Manchester, UK), with a Z-electrospray interface, set in the negative ion mode, confirmed the identities of the analytes. The collision gas was argon, and nitrogen was employed as desolvation (≅ 380 L/h) and nebulizer (≅ 38 L/ h) gas. During the analyses, the cone, capillary, and extractor were set at 20 V, 3 kV, and 3 V for the electrospray ionization source, respectively. For the confirmation of analyte identities, the multiple-reaction monitoring mode (collision energy of 15 eV) was employed in the analyses.