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Cd27 alexa fluor 488

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CD27-Alexa Fluor 488 is a fluorochrome-conjugated antibody that binds to the CD27 cell surface antigen. CD27 is a co-stimulatory molecule expressed on T cells, B cells, and natural killer cells. The Alexa Fluor 488 fluorescent dye is used to label the antibody, enabling detection and analysis of CD27-positive cells by flow cytometry.

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Lab products found in correlation

Cd14 bv510, by BioLegend (2 mentions) Igm percp cy5, by BD (2 mentions) Cd3 bv510, by BioLegend (2 mentions) Cd38 apc cy7, by BioLegend (2 mentions) Cd56 bv510, by BioLegend (2 mentions) Iga alexa fluor 647, by Jackson ImmunoResearch (2 mentions) Cd19 ecd, by Beckman Coulter (2 mentions) Facsaria iiiu, by BD (2 mentions) Igd pe cy7, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by BD (2 mentions) Cd21 bv711, by BD (1 mentions)

Spelling variants of this product

Alexa Fluor 488 (61 citations) Alexa 488 (13 citations)

2 protocols using cd27 alexa fluor 488

1

Isolation of SARS-CoV-2 Memory B Cells

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Cryopreserved PBMCs from COVID-19 convalescent donors were thawed and stained with DAPI (BD564907), CD14-BV510 (BioLegend 301842), CD3-BV510 (BioLegend 317332), CD56-BV510 (BioLegend 318340), CD19-ECD (Beckman Coulter IM2708U), CD21-BV711 (563163), IgA-Alexa Fluor 647 (Jackson Immunoresearch 109-606-011), IgD-PE-Cy7 (BD 561314), IgM-PerCP-Cy5.5 (BD561285), CD27-Alexa Fluor 488 (BioLegend 393204) and CD38-APC-Cy7 (BioLegend 303534). Stained cells were then sorted using the BD FACSAria IIIu in a BSL3 facility. This procedure involved gating out all but live CD19+CD14-CD3-CD56-IgM-IgD- cells, and then gating on IgA to yield purified populations of IgA-producing and IgG-producing memory B cells (MBCs).
Dacon C., Peng L., Lin T.H., Tucker C., Lee C.C., Cong Y., Wang L., Purser L., Cooper A.J., Williams J.K., Pyo C.W., Yuan M., Kosik I., Hu Z., Zhao M., Mohan D., Peterson M., Skinner J., Dixit S., Kollins E., Huzella L., Perry D., Byrum R., Lembirik S., Murphy M., Zhang Y., Yang E.S., Chen M., Leung K., Weinberg R.S., Pegu A., Geraghty D.E., Davidson E., Doranz B.J., Douagi I., Moir S., Yewdell J.W., Schmaljohn C., Crompton P.D., Mascola J.R., Holbrook M.R., Nemazee D., Wilson I.A, & Tan J. (2023). Rare, convergent antibodies targeting the stem helix broadly neutralize diverse betacoronaviruses. Cell Host & Microbe, 31(1), 97-111.e12.
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2

Phenotyping Memory B Cells by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cryopreserved PBMCs were thawed and stained with the following panel: DAPI (BD564907), CD14-BV510 (BioLegend, 301842), CD3-BV510 (BioLegend, 317332), CD56-BV510 (BioLegend, 318340), CD19-ECD (Beckman Coulter, IM2708U), CD21-BV711 (BD, 563163), IgA-Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-011), IgD-PE-Cy7 (BD, 561314), and IgM-PerCP-Cy5.5 (BD, 561285), CD27-Alexa Fluor 488 (BioLegend, 393204) and CD38-APC-Cy7 (BioLegend, 303534). The cells were sorted using the BD FACSAria IIIu in a BSL3 facility and gated on live CD19+CD14−CD3−CD56−IgM−IgD−IgA+/IgA− (memory B cells).
Dacon C., Tucker C., Peng L., Lee C.C., Lin T.H., Yuan M., Cong Y., Wang L., Purser L., Williams J.K., Pyo C.W., Kosik I., Hu Z., Zhao M., Mohan D., Cooper A., Peterson M., Skinner J., Dixit S., Kollins E., Huzella L., Perry D., Byrum R., Lembirik S., Zhang Y., Yang E.S., Chen M., Leung K., Weinberg R.S., Pegu A., Geraghty D.E., Davidson E., Douagi I., Moir S., Yewdell J.W., Schmaljohn C., Crompton P.D., Holbrook M.R., Nemazee D., Mascola J.R., Wilson I.A, & Tan J. (2022). Broadly neutralizing antibodies target the coronavirus fusion peptide. bioRxiv.
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