Caco-2 cells were grown in 37 °C with 5% CO2 in MEM medium containing 25 mM HEPES (Welgene, Daegu, Korea), 10% FBS (Gibco, Paisley, UK), and 1% penicillin-streptomycin antibiotics (welgene). For cell toxicity assay on AE-GBE, the cells were treated with AE-GBE at different concentrations of 50, 100, 250, 500, and 1000 μg/mL for 24 h. Then, MTT (Duchefa Biochemie BV, Haarlem, Netherlands) of 0.5 mg/mL concentration was added to each plate and further cultured for 2 h; after removal of the supernatant, insoluble formazan crystals were dissolved in 2-propanol and measured at 540 nm (TECAN Group Ltd., Shanghai, China). As another method to analyse cell viability, ATP levels in the cells were measured with the Perkin-Elmer ATPLite system (PerkinElmer Life Sciences, Boston, MA, USA), according to the manufacturer’s instructions [20 (link)]. This system was based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin. The attached mammalian cell lysis solution releases the adenine nucleotides and inactivates endogenous ATP degrading enzymes.
Penicillin streptomycin antibiotics
Manufactured by Welgene
Sourced in United Kingdom
Penicillin-streptomycin antibiotics are a combination of two commonly used antibiotic agents, penicillin and streptomycin. They are used to inhibit the growth of a wide range of bacteria in various laboratory and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hepes, by Welgene (1 mentions)
Atplite system, by PerkinElmer (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Duchefa Biochemie (1 mentions)
C2c12 myoblast, by American Type Culture Collection (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
2 protocols using penicillin streptomycin antibiotics
1
Caco-2 Cell Viability Assay with AE-GBE
2
C2C12 Myoblast Culture with Isorhamnetin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C2C12 myoblasts obtained from American Type Culture Collection (Manassa, VA, USA) were grown in Dulbecco's modified Eagle's medium (DMEM, WelGENE Inc., Daegu, Republic of Korea) supplemented with 10% fetal bovine serum (FBS) and 100 μg/ml penicillin/streptomycin antibiotics (WelGENE Inc.) in a humidified 5% CO 2 atmosphere at 37°C. Isorhamnetin (Figure 1A, Sigma-Aldrich Co., St. Louis, MO, USA) was dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich) and adjusted to the final concentrations using complete DMEM prior to use; the final DMSO concentration was <0.1% in all experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!