Primary antibodies used in immunoblot analysis were as follows: MCU (D2Z3B) rabbit mAb (CellSignaling# 14997); Bcl-2 rabbit pAb (ABclonal# A2845); Cpt1a mouse mAb (Abcam# 128568); Caspase-3(8G10) rabbit mAb (Cell Signaling# 9665); Caspase-9(C9) mouse mAb (Cell Signaling# 9508); Caspase-8 rabbit pAb (R&D Systems# af1650); Bax rabbit Ab (CellSignaling# 2772 S), Bak rabbit pAb (ABclonal# A0204); Puma rabbit pAb (ABclonal# A17138); anti-Noxa rabbit pAb (ABclonal# A9801); Cytochrome c mouse mAb (SantaCruz#sc-13156); VDAC rabbit pAb (CellSignaling# 4866); Lamin A/C rabbit pAb (CellSignaling# 2032); TGF-β1 mouse mAb (R&D Systems# MAB240); and β-actin mouse mAb (Sigma-Aldrich# A5441). All antibodies were 1:1000 diluted except the β-actin mouse mAb diluted at 1:10,000. Results were quantified using Image J.
Caspase 9 c9 mouse mab
Manufactured by Cell Signaling Technology
Sourced in United States
Caspase-9(C9) mouse mAb is a monoclonal antibody that recognizes Caspase-9, a key enzyme involved in the apoptotic signaling pathway. This antibody can be used to detect and study the expression and activation of Caspase-9 in various cellular and experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Caspase 3 8g10 rabbit mab, by Cell Signaling Technology (1 mentions)
β actin mouse mab, by Merck Group (1 mentions)
Bcl 2 rabbit pab, by ABclonal (1 mentions)
Puma rabbit pab, by ABclonal (1 mentions)
Caspase 3 3g2 mouse mab, by Cell Signaling Technology (1 mentions)
β actin 8h10d10 mouse mab, by Cell Signaling Technology (1 mentions)
Caspase 8 1c12 mouse mab, by Cell Signaling Technology (1 mentions)
Ecl prime western blotting detection reagent, by GE Healthcare (1 mentions)
Las 3000 luminescent image analyzer, by Fujifilm (1 mentions)
2 protocols using caspase 9 c9 mouse mab
1
Immunoblot Analysis of Apoptosis Regulators
2
Apoptosis Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SBC-3 cells (cell concentration: 5
× 105 cells/mL) were cultured in a six-well flat-bottom
plate and treated with EtOH/H2O (1:1), 10 μM cisplatin,
or 15 μM1 for 24 h. The Western blotting analysis
was carried out by the same procedures as previously reported.26 (link) The following antibodies were recruited: β-actin
(8H10D10 Mouse mAb, product number 3700, 1:1000; Cell Signaling Technology,
Danvers, MA, USA), caspase-8 (1C12 Mouse mAb, product number 9746,
1:1000; Cell Signaling Technology), caspase-9 (C9 Mouse mAb, product
number 9508, 1:2000; Cell Signaling Technology), caspase-3 (3G2 Mouse
mAb, product number 9668, 1:1000, Cell Signaling Technology), PARP
(46D11 Rabbit mAb, product number 9532, 1:1000; Cell Signaling Technology),
Bax (2D2 Mouse mAb, product number 89477, 1:1000; Cell Signaling Technology),
Bcl-2 (124 Mouse mAb, product number 15071, 1:1000; Cell Signaling
Technology), anti-rabbit IgG, horseradish peroxidase (HRP)-linked
antibody (product number 7074, 1:10000; Cell Signaling Technology),
and anti-mouse IgG, HRP-linked antibody (product number 7076, 1:10000;
Cell Signaling Technology). The signals were detected using ECL Prime
Western Blotting Detection Reagents (GE Healthcare, Boston, MA, USA),
and photographed by a LAS-3000 luminescent image analyzer (FUJIFILM,
Tokyo, Japan).
× 105 cells/mL) were cultured in a six-well flat-bottom
plate and treated with EtOH/H2O (1:1), 10 μM cisplatin,
or 15 μM
was carried out by the same procedures as previously reported.26 (link) The following antibodies were recruited: β-actin
(8H10D10 Mouse mAb, product number 3700, 1:1000; Cell Signaling Technology,
Danvers, MA, USA), caspase-8 (1C12 Mouse mAb, product number 9746,
1:1000; Cell Signaling Technology), caspase-9 (C9 Mouse mAb, product
number 9508, 1:2000; Cell Signaling Technology), caspase-3 (3G2 Mouse
mAb, product number 9668, 1:1000, Cell Signaling Technology), PARP
(46D11 Rabbit mAb, product number 9532, 1:1000; Cell Signaling Technology),
Bax (2D2 Mouse mAb, product number 89477, 1:1000; Cell Signaling Technology),
Bcl-2 (124 Mouse mAb, product number 15071, 1:1000; Cell Signaling
Technology), anti-rabbit IgG, horseradish peroxidase (HRP)-linked
antibody (product number 7074, 1:10000; Cell Signaling Technology),
and anti-mouse IgG, HRP-linked antibody (product number 7076, 1:10000;
Cell Signaling Technology). The signals were detected using ECL Prime
Western Blotting Detection Reagents (GE Healthcare, Boston, MA, USA),
and photographed by a LAS-3000 luminescent image analyzer (FUJIFILM,
Tokyo, Japan).
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