Rabbit anti phospho stat1 ab

Manufactured by Cell Signaling Technology

Rabbit anti-phospho-Stat1 Ab is a primary antibody that specifically recognizes the phosphorylated form of the Signal Transducer and Activator of Transcription 1 (Stat1) protein. This antibody can be used to detect and quantify the levels of activated Stat1 in various cellular and experimental systems.

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Lab products found in correlation

Rabbit anti stat1 ab, by Cell Signaling Technology (2 mentions) Amersham ecl prime western blotting detection system, by GE Healthcare (1 mentions) Rabbit anti p44 42 mapk erk1 2 ab, by Cell Signaling Technology (1 mentions) Invitrogen ibright imaging systems 1500, by Thermo Fisher Scientific (1 mentions) Nupage bis tris sds page gel, by Thermo Fisher Scientific (1 mentions) Immobilon p membrane, by Merck Group (1 mentions) Mouse anti actin antibody, by Cell Signaling Technology (1 mentions) Image studio, by LI COR (1 mentions)

Spelling variants of this product

STAT1 (305 citations) Anti-STAT1 (160 citations) Phospho-STAT1 (94 citations) Anti-phospho-STAT1 (54 citations) Rabbit anti-STAT1 (28 citations) Rabbit anti-phospho-STAT1 (6 citations)

2 protocols using rabbit anti phospho stat1 ab

Protein Extraction and Western Blot Analysis of HNSCC Cell Lines

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The total protein extraction kit for cultured animal cells and tissues (Invent Biotechnologies, Inc.) was used for protein extraction from HNSCC cell lines. Protein extracts were electrophoresed using a 4%–12% NuPAGE Bis‐Tris SDS‐PAGE gel (Invitrogen, Thermo Fisher Scientific Inc.) and transferred onto an Immobilon‐P membrane (Merck Millipore).
The membrane was then incubated with mouse anti‐human HOXB7 Ab (40–2000, 1:100 dilution, Invitrogen), rabbit anti‐phospho‐p44/42 MAPK (pERK1/2) Ab (Thr202/Tyr204; Cell Signaling Technology), rabbit anti‐p44/42 MAPK (ERK1/2) Ab (137F5; Cell Signaling Technology), mouse anti‐CIITA Ab (7‐1H; Santa Cruz Biotechnology), rabbit anti‐phospho‐Stat1 Ab (D4A7; Cell Signaling Technology), rabbit anti‐Stat1 Ab (42H3; Cell Signaling Technology), and mouse anti‐human β‐actin Ab (C4; Santa Cruz Biotechnology). The Amersham ECL Prime Western Blotting Detection System (GE Healthcare Life Sciences) and Invitrogen iBright Imaging Systems 1500 (Invitrogen) were used for detection by chemiluminescence. ImageJ was used to analyze the expression values of the target bands.²⁵

Komatsuda H., Wakisaka R., Kono M., Kumai T., Hayashi R., Yamaki H., Sato R., Nagato T., Ohkuri T., Kosaka A., Ohara K., Takahara M., Katada A, & Kobayashi H. (2022). Mitogen‐activated protein kinase inhibition augments the T cell response against HOXB7‐expressing tumor through human leukocyte antigen upregulation. Cancer Science, 114(2), 399-409.

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Immunoblotting Analysis of Naïve CD4+ T Cells

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Western blotting analyses were performed with whole cell lysates of antagomiR-31 or antgoNC treated naïve CD4+ T cells according to previously published procedures (25 (link)). Following antibodies were used: mouse anti-actin antibody (1:3,000; Cell Signaling Technology, #3700S), rabbit anti-STAT1 Ab (1:1,000; Cell Signaling Technology, #9172S), and rabbit anti-phospho-STAT1 Ab (Cell Signaling Technology, #9167S). The immunoblots was imaged and analyzed using Image Studio (LI-COR Biosciences). The anti-human IFN-γ antibody (clone B27) used for neutralization of IFN-γ in the cell culture medium and the IgG1κ isotype control antibody (Clone MG1-45, Cat. No. 401409) were purchased from BioLegend.

Zhu L., Qiu C., Dai L., Zhang L., Feng M., Yang Y., Qiu C., Zhang A., Huang J., Wang Y., Wan Y., Zhao C., Wu H., Lyu J., Zhang X, & Xu J. (2021). Hsa-miR-31 Governs T-Cell Homeostasis in HIV Protection via IFN-γ-Stat1-T-Bet Axis. Frontiers in Immunology, 12, 771279.

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