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Mouse anti chondroitin sulfate antibody cs 56

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The Mouse anti-chondroitin sulfate antibody (CS-56) is a laboratory reagent used for the detection and identification of chondroitin sulfate in biological samples. It is a monoclonal antibody that specifically binds to chondroitin sulfate, a type of glycosaminoglycan found in the extracellular matrix and on cell surfaces.

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Lab products found in correlation

Nitrocellulose membrane, by Thermo Fisher Scientific (2 mentions) Hrp conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Rabbit anti fibronectin, by Merck Group (2 mentions) Rabbit anti laminin 1, by Merck Group (2 mentions) Rabbit anti collagen 1a1, by Novus Biologicals (2 mentions) Lds lithium dodecyl sulfate buffer, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Chondroitin sulfate (134 citations) Chondroitin sulfate C (24 citations) CS-56 (14 citations) Mouse anti-chondroitin sulfate (2 citations)

2 protocols using mouse anti chondroitin sulfate antibody cs 56

1

Dot Blot Immunoassay of ECM Proteins

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For dot blot immunoassay of laminin 1, fibronectin, collagen 1 or CSPG, spinal cord tissue blocks were lysed and homogenized in standard RIPA (radio-immuno-precipitation-assay) buffer. LDS (lithium dodecyl sulfate) buffer (Life Technologies) was added to the post-mitochondrial supernatant and 2μL containing 2μg/uL protein was spotted onto a nitrocellulose membrane (Life Technologies), set to dry and incubated overnight with primary antibodies: rabbit anti-Laminin 1 (1:4000, Sigma, St.Louis, MO); rabbit anti-Fibronectin (1:7000, Millipore, Burlington, MA); rabbit anti-Collagen 1a1 (1:7000, Novus Biologicals, Littleton, CO); mouse anti-chondroitin sulfate antibody (CS-56, 1:3000, Sigma Aldrich), an IgM-monoclonal antibody that detects glyco-moieties of all CSPGs40 (link). Immunoreactivity was detected on X-ray film with horse radish peroxidase (HRP)-conjugated secondary antibody (1:5000) and chemiluminescent substrate (ThermoFisher, Grand Ilsand, NY). Densitometry measurements of immunoreactivity were obtained using ImageJ software (NIH) and normalized to total protein (Ponceau S) density43 (link). Raw images of dot blots are provided as Supplementary figure 1.
Anderson M.A., O’Shea T.M., Burda J.E., Ao Y., Barlatey S.L., Bernstein A.M., Kim J.H., James N.D., Rogers A., Kato B., Wollenberg A.L., Kawaguchi R., Coppola G., Wang C., Deming T.J., He Z., Courtine G, & Sofroniew M.V. (2018). Required growth facilitators propel axon regeneration across complete spinal cord injury. Nature, 561(7723), 396-400.
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2

Dot Blot Immunoassay of ECM Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
For dot blot immunoassay of laminin 1, fibronectin, collagen 1 or CSPG, spinal cord tissue blocks were lysed and homogenized in standard RIPA (radio-immuno-precipitation-assay) buffer. LDS (lithium dodecyl sulfate) buffer (Life Technologies) was added to the post-mitochondrial supernatant and 2μL containing 2μg/uL protein was spotted onto a nitrocellulose membrane (Life Technologies), set to dry and incubated overnight with primary antibodies: rabbit anti-Laminin 1 (1:4000, Sigma, St.Louis, MO); rabbit anti-Fibronectin (1:7000, Millipore, Burlington, MA); rabbit anti-Collagen 1a1 (1:7000, Novus Biologicals, Littleton, CO); mouse anti-chondroitin sulfate antibody (CS-56, 1:3000, Sigma Aldrich), an IgM-monoclonal antibody that detects glyco-moieties of all CSPGs40 (link). Immunoreactivity was detected on X-ray film with horse radish peroxidase (HRP)-conjugated secondary antibody (1:5000) and chemiluminescent substrate (ThermoFisher, Grand Ilsand, NY). Densitometry measurements of immunoreactivity were obtained using ImageJ software (NIH) and normalized to total protein (Ponceau S) density43 (link). Raw images of dot blots are provided as Supplementary figure 1.
Anderson M.A., O’Shea T.M., Burda J.E., Ao Y., Barlatey S.L., Bernstein A.M., Kim J.H., James N.D., Rogers A., Kato B., Wollenberg A.L., Kawaguchi R., Coppola G., Wang C., Deming T.J., He Z., Courtine G, & Sofroniew M.V. (2018). Required growth facilitators propel axon regeneration across complete spinal cord injury. Nature, 561(7723), 396-400.
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