Tissue sections were stained with hematoxylin and eosin (HE). Parasagittal sections of cerebellum were scanned using an Olympus BX61VS microscope with UPlanSApo 20x/0.75 objective and TIFF images were acquired using the Olympus dotSlide system (Olympus, Tokyo, Japan). All those PCs were counted in which a nucleus with a nucleolus was observed. The PC layer was traced and length of the trace was measured using ImageProPlus 7.0 software (Media Cybernetics, Rockville, MD, USA). The number of PCs per unit of PC layer was quantified for the anterior, central, posterior, and flocculonodular transversal zones of the cerebellum [21 (link)].
Uplansapo 20x 0.75 objective
Manufactured by Olympus
Sourced in Japan
The UPlanSApo 20x/0.75 objective is a high-numerical aperture objective designed for Olympus microscopes. It provides a magnification of 20x and a numerical aperture of 0.75, which enables high-resolution imaging.
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Lab products found in correlation
Bx61vs microscope, by Olympus (1 mentions)
Image pro plus 7, by Media Cybernetics (1 mentions)
Dotslide system, by Olympus (1 mentions)
Rabbit polyclonal antibodies against p65, by Santa Cruz Biotechnology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 phalloidin, by Thermo Fisher Scientific (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Ix71 microscope, by Olympus (1 mentions)
2 protocols using uplansapo 20x 0.75 objective
1
Quantification of Purkinje Cells in Cerebellum
2
Immunostaining of IL-1β-Stimulated HUVECs
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Post-confluent HUVECs cultured on fibronectin-coated glass coverslips were treated with comfrey or TAK inhibitor for 30 min before IL-1β stimulation. At the indicated time points, cells were fixed for 15 min with 4% paraformaldehyde (Sigma), permeable for 30 min with 0.1% Triton X-100 (Sigma), washed with PBS, and finally blocked for 1 h with 3% BSA-TBS-T. For immunostaining, rabbit polyclonal antibodies against p65 (Santa Cruz) (1:500) were used with a secondary antibody conjugated to an Alexa-Fluor 488 goat antibody against rabbit IgG antibody (Invitrogen) in a ratio of 1:1000. Cells were contrasted for 15 min with Alexa Fluor 568 phalloidin (1:1000) and 5 min with 4',6-diamidino-2phenylindole (DAPI, Life Technologies; 1:10,000). The samples were examined using an Olympus IX71 microscope with a UPlanSApo 20x/0.75 objective. The images were processed using the ImageJ program.
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