All in vitro kinase assays were done in NEBuffer for protein kinase (NEB) at 30°C. As a phosphate donor, 5 mM ATP or GTP was used. Recombinant Snf1 catalytic domain (Snf1-CAT) was phosphorylated by 1.4 pmol of recombinant human CaMKK2 (Abnova) for 2 hr at 30°C for its activation (Figure 5F ). When Tda1 fragments were used as kinases (Figure 4F ), additional DTT was supplemented up to 1 mM. The reactions were quenched by SDS sample buffer addition, then boiled at 100°C for 5 min.
Recombinant human camkk2
Manufactured by Abnova
Recombinant human CaMKK2 is a protein produced using recombinant DNA technology. CaMKK2 is a calmodulin-dependent protein kinase kinase that plays a role in the calcium/calmodulin-dependent protein kinase (CaMK) signaling pathway.
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Lab products found in correlation
2 protocols using recombinant human camkk2
1
Phosphorylation of Snf1 Kinase Catalytic Domain
2
Snf1 Kinase Activation Assay
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All in vitro kinase assays were done in NEBuffer for protein kinase (NEB) at 30°C. Recombinant Snf1 kinase domain (Snf1-KD) was phosphorylated by recombinant human CaMKK2 (Abnova) for its activation before kinase assays (Figure 5F). When Tda1 fragments were used as kinases (Figure . 4F), additional DTT was supplemented up to 1 mM. The reactions were quenched by SDS sample buffer addition, then boiled at 100°C for 5 minutes.
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