Anti mouse cd41 pe

Platelets isolated from either 13-week-old WT and PyMT mice were pooled, and 2 × 10⁸ cells were injected via the left lateral tail vein into recipient Mpl^−/− mice. Platelet counts were assessed before and 2 hours after transfusion by staining whole blood with anti-mouse CD41 PE (BioLegend, 148304). Two hours after platelet transfusion, mice were injected via the right lateral tail vein with 2.5 × 10⁵ CellTracker™ Green CMFDA-labeled (Invitrogen, C2925) AT-3 tumor cells. Mice were sacrificed 48 hours after tumor cell injection, and tumor colonization was assessed by digesting lung tissue in collagenase/hyaluronidase (STEMCELL Technologies, 07912) and quantifying the number of CMFDA⁺ cells using a Cytek Aurora spectral cytometer.

Endogenous platelet survival was measured by labeling Sulfo–NHS–LC-Biotin (Pierce, Beijing, China) as previously [28 (link)]. In brief, 2 h after tail intravenous injection of 100 mg/kg Sulfo–NHS–LC-Biotin, 200 TCID₅₀ SFTSV intravenously injected into IFNar^−/− mice. Blood (10 μL) from the facial vein collected at different time points, after anti-mouse CD41-PE (Biolegend, San Diego, CA, USA) and PE-Cy7-Streptavidin staining (Biolegend, #405206), the biotin-labeled platelets were detected by flow cytometry.