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Opteia set mouse mcp 1

Manufactured by BD
Sourced in United States, Japan

The BD OptEIA™ Set Mouse MCP-1 is a specific enzyme-linked immunosorbent assay (ELISA) kit designed to quantify mouse monocyte chemoattractant protein-1 (MCP-1) levels in biological samples.

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2 protocols using opteia set mouse mcp 1

1

Cytokine Profiling of Serum Samples

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Whole blood was collected into test tubes and allowed to clot at room temperature for 30 min. The clot was removed by centrifuging at 1,000–2,000 × g for 10 minutes in a refrigerated centrifuge. The collected serum was stored at −80°C until further analysis. The concentrations of IL-6, TNF-α, and MCP1 in the sera were measured using commercially available ELISA assays (BD OptEIA™ Set Mouse IL-6; BD OptEIA™ Set Mouse TNF-alpha; BD OptEIA™ Set Mouse MCP-1; BD Biosciences, San Diego, USA), according to the manufacturer’s recommendations.
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2

Cytokine and Chemokine Quantification

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The plasma concentrations of the cytokines, including interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ, were measured by using a cytokine bead array kit (BD Bioscience, Tokyo, Japan). The plasma level of monocyte chemotactic protein-1 (MCP-1) was quantified by means of enzyme-linked immunosorbent assay (BD OptEIA Set Mouse MCP-1; BD Biosciences, Tokyo, Japan). These assays were performed as described in the manufacturer's protocol.
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