Secreted DKK1 levels were measured either by Western blot after immunoprecipitating DKK1 from the culture medium using anti-DKK1 (#AF1096; R&D Systems) or by commercially available ELISA assay (Human Dkk-1 Quantikine ELISA Kit, #DKK100B; R&D Systems) according to the manufacturer’s protocol. For DKK1 ELISA, absorbance was measured at 450 nm with a correction at 540 nm using the Eon microplate spectrophotometer (Agilent).
Anti dkk1
Manufactured by R&D Systems
Anti-DKK1 is a laboratory reagent used in research applications. It is an antibody that specifically binds to the Dickkopf-related protein 1 (DKK1), a secreted protein involved in the regulation of various cellular processes. The primary function of Anti-DKK1 is to facilitate the detection and quantification of DKK1 in biological samples through techniques such as ELISA, Western blotting, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Af1096, by R&D Systems (2 mentions)
Human dkk1 quantikine elisa kit, by R&D Systems (1 mentions)
Eon microplate spectrophotometer, by Agilent Technologies (1 mentions)
Odyssey v3, by LI COR (1 mentions)
Irdye 680 and 800, by LI COR (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
2 protocols using anti dkk1
1
Quantitative DKK1 Measurement Protocols
2
Western Blot Analysis of SFRP1 and DKK1
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SDS–PAGE gels were blotted onto nitrocellulose membranes and incubated with the respective primary antibodies (anti-SFRP1, Cell Signaling Technologies, clone D5A7, 1:500; anti-DKK1, R&D Systems, AF1096, 1:2,000). As secondary antibodies fluorescently labelled IRDye800 and 680 (LI-COR, Lincoln, NE) were used. Fluorescence detection was performed using an Odyssey infrared imaging system (LI-COR) and blots were further analysed using the Odyssey v3.0 software (LI-COR).
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