Block solution

Manufactured by Merck Group

Block solution is a laboratory reagent used to prevent non-specific binding in immunoassays and other protein-based analyses. It is designed to block unoccupied binding sites on solid supports, reducing background signals and improving the specificity of detection.

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Lab products found in correlation

Mouse anti βiii tubulin antibody, by Promega (1 mentions) Ncl ki67p, by Leica Biosystems (1 mentions) Alexa fluor conjugated goat anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions) Dm2500, by Leica Microsystems (1 mentions)

2 protocols using block solution

Immunocytochemistry of DRG Neurons

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Cells were fixed with 4% paraformaldehyde for 30 minutes,
and then washed 3 times in PBST (PBS + 0.01%Triton X). The
coverslips were placed in block solution (1xPBS + 5%FBS + 0.2%Triton
X (Sigma-Aldrich, St. Louis, MO) for 30 minutes. DRG neurons were
stained using mouse anti-βIII tubulin antibody (Promega Co.,
Madison, WI), and Alexa Fluor 594 donkey anti-mouse secondary IgG
antibody (Life Technologies, Carlsbad, CA), and placed on
Fluoromount G medium (EM laboratories Inc., Electron Microscopy
Sciences, Cat# 17984–25) for observation.

Patel N.J., Hogan K.J., Rizk E., Stewart K., Madrid A., Vadakkadath Meethal S., Alisch R., Borth L., Papale L.A., Ondoma S., Gorges L.R., Weber K., Lake W., Bauer A., Hariharan N., Kuehn T., Cook T., Keles S., Newton M.A, & Iskandar B.J. (2020). Ancestral Folate Promotes Neuronal Regeneration in Serial Generations of Progeny. Molecular neurobiology, 57(4), 2048-2071.

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Immunofluorescent Labeling of Ki67

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The HMCs were washed with PBS for 10 min and fixed with 4% paraformaldehyde for 20 min after drying, washed with PBS containing 0.1% Triton for 10 min, sealed with Block solution (11921673001, Sigma-Aldrich; Merck KGaA) for 30 min, incubated with a rabbit anti-Ki67 primary antibody (1:200 diluted with Block solution; NCL-Ki67p, Novocastra Laboratories, British) at 4°C over-night, washed with PBS without Triton 3 times for 5 min each, incubated with Alexa Fluor conjugated goat anti-rabbit secondary antibody (A32731, Invitrogen; Thermo Fisher Scientific, Inc.) in the dark for 1 h, washed with PBS 3 times (5 min each), and immediately observed under a fluorescence microscope (DM2500, Leica Microsystems GmbH).

Gao Y., Yang H., Wang Y., Tian J., Li R, & Zhou X. (2020). Evaluation of the inhibitory effect of tacrolimus combined with mycophenolate mofetil on mesangial cell proliferation based on the cell cycle. International Journal of Molecular Medicine, 46(4), 1582-1592.

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