The largest database of trusted experimental protocols

Tetramethylbenzidine substrate

Manufactured by Tiangen Biotech
Sourced in China

Tetramethylbenzidine (TMB) substrate is a chromogenic substrate used in various immunoassays, such as enzyme-linked immunosorbent assays (ELISA). It serves as a colorimetric indicator, producing a blue color upon oxidation by peroxidase enzymes. The intensity of the blue color is proportional to the amount of the target analyte present in the sample.

Automatically generated - may contain errors

2 protocols using tetramethylbenzidine substrate

1

SARS-CoV-2 Antibody Detection by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
SARS-CoV-2 NP and S-RBD specific IgG antibodies in plasma were measured by in-house ELISA separately. Purified NP and S-RBD antigens were coated onto MaxiSorp ELISA plates (Corning Costar, Acton, MA, USA) in 0.1 M carbonate buffer (pH 9·6) at concentration of 0.2 μg/mL overnight at 4 °C, respectively. Plates were washed 4 times with phosphate-buffered saline (PBS) containing 0.1% vol/vol Tween-20 (PBST) and blocked with 1% bovine serum albumin for 2 h at 37 °C. The plates were then washed with PBST. The serum samples were diluted to 80-fold into PBS as initial concentration, and then serial 2-fold diluted until 81920-fold. The serial dilutions of serum samples were added to the plate wells and incubated, followed by wash and incubation with anti-human IgG HRP-conjugated coat secondary antibody (Abcam, Cambridge, UK). After 4 washes, plates were developed by tetramethylbenzidine substrate (TianGen Biotech Co., Beijing, China) at room temperature in the dark. The absorbance was measured at 450 nm using a microplate reader (Molecular Devices Co., Sunnyvale, CA, USA) after adding the stop solution (2 M sulphuric acid). All samples were run in duplicate. The titers of NP and S-RBD specific IgG antibodies were defined as the highest dilution giving an absorbance value of more than 2.1 times that of the negative control.
+ Open protocol
+ Expand
2

ELISA for Chicken Adenovirus Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
ELISA plates (Guangzhou Jet Bio-Filtration Co., Ltd., China) were coated with 0.2 µg (each)/well of mixed purified fiber-1, fiber-2, and penton (expressed in E. coli and stored by our laboratory).
Then, the plates were reacted with sera from chickens immunized with the corresponding proteins.
Commercial peroxidase-conjugated rabbit anti-chicken IgG (Sigma) was used as the secondary antibody. Following incubation with tetra-methylbenzidine substrate (Tiangen Biotech (Beijing) Co., ltd., China), the reaction was stopped with 0.5 M sulfuric acid and the optical density of each well was measured at a wavelength of 450 nm.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!