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Nimblegen labeling kit

Manufactured by Roche
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The NimbleGen labeling kit is a laboratory product designed for labeling nucleic acid samples. It provides the necessary reagents and tools for labeling DNA or RNA samples in preparation for downstream processing or analysis. The kit contains the essential components required for the labeling process, allowing users to efficiently and accurately label their samples.

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Lab products found in correlation

400k array platform, by Agilent Technologies (2 mentions) Cytogenomics 4, by Agilent Technologies (2 mentions) Feature extraction, by Agilent Technologies (2 mentions)

Spelling variants of this product

NimbleGen One-Color DNA Labeling Kit (11 citations) NimbleGen Dual-ColorDNA Labeling Kit (3 citations) NimbleGen Dual-Color Labeling Kit (3 citations) NimbleGen One-Color labeling kit (2 citations)

2 protocols using nimblegen labeling kit

1

Array-based Identification of Rare CNVs in ID/DD

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Array labeling and hybridization was performed as previously described76 (link). Briefly, 250 ng of sample DNA was labeled with Cy3 using a NimbleGen labeling kit (Roche). Reference DNA (NA12878) was labeled in a pooled reaction for four arrays with Cy5 using 1 ug of DNA. Hybridization was performed using the Agilent 2×400K array platform using standard reagents, imaged using an Agilent Scanner, and processed using Agilent Feature Extraction. CNV calls were generated using Agilent CytoGenomics 4.03.12 and the ADM2 calling algorithm with default parameters. For samples passing standard Agilent QC parameters (DLRSD < 0.2), all CNVs over 100 kbp were visually inspected, filtered for known reference sample artifacts, and compared to 29,085 cases of ID/DD and 19,584 controls77 to identify rare CNVs that may contribute to pathogenicity in these cases.
Geisheker M.R., Heymann G., Wang T., Coe B.P., Turner T.N., Stessman H.A., Hoekzema K., Kvarnung M., Shaw M., Friend K., Liebelt J., Barnett C., Thompson E.M., Haan E., Guo H., Anderlid B.M., Nordgren A., Lindstrand A., Vandeweyer G., Alberti A., Avola E., Vinci M., Giusto S., Pramparo T., Pierce K., Nalabolu S., Michaelson J.J., Sedlacek Z., Santen G.W., Peeters H., Hakonarson H., Courchesne E., Romano C., Kooy R.F., Bernier R.A., Nordenskjöld M., Gecz J., Xia K., Zweifel L.S, & Eichler E.E. (2017). Hotspots of missense mutation identify novel neurodevelopmental disorder genes and functional domains. Nature neuroscience, 20(8), 1043-1051.
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2

Array-based Identification of Rare CNVs in ID/DD

Check if the same lab product or an alternative is used in the 5 most similar protocols
Array labeling and hybridization was performed as previously described76 (link). Briefly, 250 ng of sample DNA was labeled with Cy3 using a NimbleGen labeling kit (Roche). Reference DNA (NA12878) was labeled in a pooled reaction for four arrays with Cy5 using 1 ug of DNA. Hybridization was performed using the Agilent 2×400K array platform using standard reagents, imaged using an Agilent Scanner, and processed using Agilent Feature Extraction. CNV calls were generated using Agilent CytoGenomics 4.03.12 and the ADM2 calling algorithm with default parameters. For samples passing standard Agilent QC parameters (DLRSD < 0.2), all CNVs over 100 kbp were visually inspected, filtered for known reference sample artifacts, and compared to 29,085 cases of ID/DD and 19,584 controls77 to identify rare CNVs that may contribute to pathogenicity in these cases.
Geisheker M.R., Heymann G., Wang T., Coe B.P., Turner T.N., Stessman H.A., Hoekzema K., Kvarnung M., Shaw M., Friend K., Liebelt J., Barnett C., Thompson E.M., Haan E., Guo H., Anderlid B.M., Nordgren A., Lindstrand A., Vandeweyer G., Alberti A., Avola E., Vinci M., Giusto S., Pramparo T., Pierce K., Nalabolu S., Michaelson J.J., Sedlacek Z., Santen G.W., Peeters H., Hakonarson H., Courchesne E., Romano C., Kooy R.F., Bernier R.A., Nordenskjöld M., Gecz J., Xia K., Zweifel L.S, & Eichler E.E. (2017). Hotspots of missense mutation identify novel neurodevelopmental disorder genes and functional domains. Nature neuroscience, 20(8), 1043-1051.
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