Protein A/G is a recombinant protein expressed in Escherichia coli (50.5 kDa; 6 IgG binding sites) and is recommended for purifying polyclonal IgG from many species. Polyclonal mouse IgG from the serum of 7-9-week-old C57BL/6, non-lupus-prone MRL/+ mice and lupus-prone MRL/lpr mice or 6-7-month-old lupus-prone MRL/lpr mice was purified by Pierce Recombinant Protein A/G (Thermo Scientific Cat #21186). 100 μg IgG per mouse were i.v. injected into 7-9-week-old non-lupus-prone mice. PBS was used as the control.
Pierce recombinant protein a g
Manufactured by Thermo Fisher Scientific
Pierce Recombinant Protein A/G is a hybrid protein that combines the IgG-binding domains of Protein A and Protein G. It is designed for the purification of a wide range of antibody types, including IgG, IgM, IgA, IgE, and IgD, from various species. The product is intended for laboratory use and research purposes.
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Lab products found in correlation
2 protocols using pierce recombinant protein a g
1
Polyclonal IgG Purification and Transfer
2
Protein A/G Microarray Fabrication
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The microarray test sample was fabricated using the commercially available Pierce Recombinant Protein A/G (Thermo Fisher Scientific, 21186). First, the stock solution at 5 mg/ml was diluted using Milli-Q water to a final concentration of 500 μg/ml. This was then spotted (using a SCIENION sciFLEXARRAYER S3 spotter) onto a borosilicate glass slide (NEXTERION Slide E, SCHOTT), coated with a multipurpose epoxysilane layer that covalently binds most types of biomolecules including amino- and nonmodified DNA, RNA, and proteins. Spots of diameter 500 μm were made with 1000-μm center-to-center spacing, and the sample was left to dry overnight (24 hours) before measuring.
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