1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc hcl

Materials. Triethylamine (TEA, 99%), trimethylolethane (98%), 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC•HCl, 98%), 4-methoxyphenol (99%), acryloyl chloride (95%), propargyl alcohol (98%), lithium bromide (LiBr, 99%) and N,Ndimethylacrylamide (DMA, 99%) were purchased from Tokyo Chemical Industry (Tokyo, Japan). 4-Dimethylaminopyridine (DMAP, 99%), 2,2'-azobis[2-(2-imidazolin-2-yl)propane] dihydrochloride (AIPD, 98%) and formic acid (99%) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Diethylether (Et2O), N,N-dimethylformamide (DMF), copper(II) sulfate (CuSO4, 97.5%), and sodium L-ascorbate (98%) were purchased from Kanto Chemical (Tokyo, Japan). Acetonitrile (MeCN) and fetuin from fetal bovine serum were purchased from Sigma Aldrich (St. Louis, USA). Blood cell suspension from a chicken was purchased from Nippon Bio-test Laboratory Inc (Saitama, Japan). 2-{[(Butylsulfanyl)carbonothioyl]sulfanyl}propanoic acid (BTPA), 36 6'-sialyllactose azide (6'-SALac azide), 37 and trifunctional RAFT agent 35 were prepared according to previous papers. Commercial monomers including the radical inhibitor were purified by passing through an alumina column prior to use.