Dspe peg2k

Hydrogenated
soybean phosphatidylcholine
(HSPC), cholesterol, and DSPE-PEG2k were obtained from Lipoid (Ludwigshafen,
Germany). For more details on these lipids, see Wei et al.^{9 (link)} Doxorubicin hydrochloride was obtained from Sicor,
Rho, Italy. Ammonium sulfate, ammonium chloride, sodium sulfate, and
USP grade l-histidine were obtained from Merck (Darmstadt,
Germany). Analytical grade sucrose was obtained from Bio-Lab Ltd.
(Jerusalem, Israel). All of the other reagents used were of analytical
grade or better. High-purity water of conductivity 18.2 MΩ was
prepared using the Barnstead Nanopure ultrapure water purification
system (Thermo Fisher Scientific, Waltham, MA) and referred as deionized
distilled water. Lipodox is a product of Sun Pharma (Gujarat, India).
All of the DSC studies of Lipodox were performed before the product
expiration date.

The
lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
(DPPC), cholesterol, 1,2-dioleoyl-3-trimethylammonium-propane
(chloride salt) (DOTAP), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt) (DOPE-PEG2k)
were supplied from Avanti Polar Lipids. The lipophilic carbocyanine
dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine
perchlorate (DiI) was supplied from Thermo Fisher Scientific. The
CpG ODN 2395 was supplied from Invivogen. The stealth liposome mix
(consisting of hydrogenated soy l-α-phosphatidylcholine
(HSPC), cholesterol, and DSPE-PEG2k in a weight ratio of 3:1:1) was
acquired from Lipoid GmbH (Germany). Reagents for the buffers used
for preparation of the NPs (phosphate-buffered saline (PBS), sodium
chloride, sodium citrate, and Hepes, glucose, and sodium cholate hydrate),
99.9% absolute ethanol, and tert-Butanol were all
supplied from Sigma-Aldrich (Denmark).