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Zymed bcl 2 elisa kit

Manufactured by Thermo Fisher Scientific
Sourced in Germany

The Zymed Bcl-2 ELISA Kit is a quantitative enzyme-linked immunosorbent assay designed to measure the levels of Bcl-2 protein in biological samples. Bcl-2 is a key regulator of apoptosis, or programmed cell death. The kit utilizes a specific antibody to detect and quantify Bcl-2 levels in the sample.

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2 protocols using zymed bcl 2 elisa kit

1

Apoptosis Regulation in HepG2 Cells

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HepG2 cancer cells were grown in DMEM containing 5% FBS at 37°C, and after treatment with the present marine extracts, HepG2 suspension was tested for Bax and Bcl-2 using cell extraction buffer to be lysed. This lysate was diluted in the standard diluent buffer over the range of the assay and measured for human active Bax and Bcl-2 content using Bax ELISA (EIA-4487) kit from DRG Instruments, Ma, Germany, and Zymed Bcl-2 ELISA Kit from Thermo Fisher Scientific Co., Waltham, MA, USA.
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2

Evaluating Apoptosis Markers in Marine Extract-Treated Cancer Cells

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Cancer cells were grown in DMEM containing 5% FBS at 37°C and after treatment with present marine extracts, cancer cells suspension was tested for Bax and Bcl-2 using lysed cell extraction. This cell lysate was diluted in standard diluent buffer over the range of the assay and measured for human active Bax and Bcl-2 content using Bax ELISA (EIA-4487) kit (DRG Instruments, Ma, Germany) and Zymed Bcl-2 ELISA Kit (Thermofisher Scientific Co., Waltham, MA, USA).
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